Naslov
Molecular diversity of Epstein-Barr virus

Autori
Rozman, Marija ; Bodulić, Kristian ; Židovec- Lepej, Snježana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
6. Simpozij studenata doktorskih studija PMF-a, Knjiga sažetaka / Schneider, Petra - Zagreb : Prirodoslovno-matematički fakultet Sveučilišta u Zagrebu, 2022, 90-91

ISBN
978-953-6076-93-2

Skup
6. Simpozij studenata doktorskih studija PMF-a = 6th Faculty of Science PhD Student Symposium

Mjesto i datum
Zagreb, Hrvatska, 23.04.2022. - 24.04.2022

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
Epstein-Barr virus ; EBV ; Mononucleosis ; Molecular diversity ; LMP1

Sažetak
Epstein-Barr virus (EBV) is an ubiquitous virus that belongs to the Herpesviridae family. Acute EBV infection in adolescents and young adults often presents as infectious mononucleosis (IM). Replication cycle of EBV involves a lysogenic phase which is characterized by latent infection and a lytic phase during which new virions are produced. EBV latent infection is classified into 5 types (0, I, IIA, IIB, III) and can be associated with the development of malignant diseases as result of neoplastic transformation of epithelial cells, lymphocytes and mezenhimal cells. Latency stages type IIA and III are characterised by the expression of an LMP-1 gene that is the most significant oncogene of EBV. LMP-1 protein imitates a biological activity of CD40 molecule which leads to transformation of Blymphocytes into proliferating lymphoblastoid cells. EBV isolates are classified into six LMP-1 variants defined by specific mutations in C- terminal region of LMP-1 gene: China1, China2, North Carolina, Alaskan, Mediterranean with deletion and Mediterranean without deletion [2]. The aim of this study was to analyse molecular diversity of EBV based on LMP-1 polymorphisms in pediatric patients with IM. In this study peripheral blood samples, from 34 children with IM (median age 12 years) with >1000 copies of EBV DNA/mL, were used. By applying specifically designed primers and polymerase chain reaction, target sequences of LMP-1 C-terminal region were amplified and analysed with Sanger sequencing. Obtained sequences were compared with a reference sequence (strain: B95-8, GenBank accession number: NC_007605) and analysed with bioinformatics alignment tools. In total 14/34 sequences were wild type, 6/34 North Carolina and 3/34 Mediterranean without deletion. Coinfections with wild type and North Carolina were detected in 4/34 patients while 7 samples showed coinfection with wild type and China1. Mann- Whitney U test was used to define the relationship between LMP-1 variants and selected immunological parameters. Concentrations of an inflammatory biomarker C-reactive protein was significantly higher in patients with coinfection of LMP-1 variants (median 13 mg/L) compared to patients infected with a single variant (median 9, 25 mg/L, p=0, 048). The ratio of CD4+/CD8+ T- lymphocytes was higher in coinfected patients (0, 3) compared with patients infected with a single variant (0, 2, p=0, 05). The results of this study showed a broad range of LMP-1 genetic diversity in IM and suggested a possible association between coinfection with different LMP-1 variants and parameters of cellular and inflammatory immunological response.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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