Pregled bibliografske jedinice broj: 1219749
Preliminary study results on the gene expression analysis of intramuscular fat content in Black Slavonian pig
Preliminary study results on the gene expression analysis of intramuscular fat content in Black Slavonian pig // Book of Abstracts of the International Symposium 29th Animal Science days
Zadar, Hrvatska, 2022. str. 33-33 (predavanje, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 1219749 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Preliminary study results on the gene expression
analysis of intramuscular fat content in Black
Slavonian pig
Autori
Lipavić, Goran ; Raguz, Nikola ; Curik, Ino ; Cubric-Curik, Vlatka ; Lukic, Boris
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Book of Abstracts of the International Symposium 29th Animal Science days
/ - , 2022, 33-33
Skup
30th International Symposium Animal Science Days (ASD 2022)
Mjesto i datum
Zadar, Hrvatska, 21.09.2022. - 23.09.2022
Vrsta sudjelovanja
Predavanje
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
Gene expression ; intramuscular fat content ; Black Slavonian pig ; genomics
Sažetak
Intramuscular fat (IMF) plays an important role in determination of meat quality and directly contributes to the positive technological and sensory characteristics such as taste, texture and juiciness. In Black Slavonian pig (BS), numerous studies reported much higher IMF content compared to other local breeds, therefore, understanding the genetic mechanisms of IMF in BS becomes one of the main challenges in scientific research. Transcriptome and gene expression analyses using RNA-seq technology is approach which enables the identification of genes that are differentially expressed in distinct phenotypes. In the last few years, the majority of gene expression studies has been carried out on modern pig breeds, therefore such analyses on local breeds would significantly contribute to the pig breeding of local breeds, but also to the commercial breeding programs. For the analysis of gene expression for IMF in this study, out of the total of 80 pigs in full-sib design, 10 sib pairs with extremely high and low IMF phenotypes were selected, and also analyzed for performance and meat quality traits. Whole transcriptome analysis was performed using RNA-seq technology, while candidate genes were further verified using quantitative real-time PCR (qPCR) analysis. The results from this study will certainly contribute to the understanding of genetic mechanisms of IMF in BS, and will also provide new possibilities for improvement of breeding program.
Izvorni jezik
Engleski
Znanstvena područja
Poljoprivreda (agronomija), Interdisciplinarne biotehničke znanosti
POVEZANOST RADA
