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Pregled bibliografske jedinice broj: 1219621

THE ROLE AND DIVERSITY OF PIR PROTEINS IN CELL WALLS OF BUDDING YEASTS


Žunar, Bojan; Lozančić, Mateja; Martinić-Cezar, Tea; Teparić, Renata; Mrša, Vladimir
THE ROLE AND DIVERSITY OF PIR PROTEINS IN CELL WALLS OF BUDDING YEASTS // HDBMB22 From Science to Knowledge / Dulić, Morana ; Sinčić, Nino ; Vrhovac Madunić, Ivana (ur.).
Zagreb: Hrvatsko Društvo za Biotehnologiju, 2022. str. 165-165 (poster, nije recenziran, sažetak, znanstveni)


CROSBI ID: 1219621 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
THE ROLE AND DIVERSITY OF PIR PROTEINS IN CELL WALLS OF BUDDING YEASTS

Autori
Žunar, Bojan ; Lozančić, Mateja ; Martinić-Cezar, Tea ; Teparić, Renata ; Mrša, Vladimir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
HDBMB22 From Science to Knowledge / Dulić, Morana ; Sinčić, Nino ; Vrhovac Madunić, Ivana - Zagreb : Hrvatsko Društvo za Biotehnologiju, 2022, 165-165

Skup
Congress of the Croatian Society of Biochemistry and Molecular Biology: From Science to Knowledge (HDBMB22)

Mjesto i datum
Brela, Hrvatska, 28.09.2022. - 01.10.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Pir proteins, Alphafold2, in silico prediction of protein structures

Sažetak
Pir proteins (proteins with internal repeats) are the most prominent proteins that yeast Saccharomyces cerevisiae covalently binds to its cell wall through β-1, 3-glucan residues. As such, they are regularly used for yeast surface display when the displayed protein needs to be bound close to its N-termini. However, the role of Pir proteins in cells remains unclear, and their diversity in closely-related species unexplored. These unknowns hamper rational engineering of Pir- based surface display, a technique with the potential to convert the entire yeast cell wall into enzymatically active self-regenerating living material. To address this issue, we used a deep- learning Alphafold2-based algorithm to in silico predict the 3D protein structures of S. cerevisiae Pir proteins and compare them with structures deposited in the Protein Data Bank (PBD). Through this approach, we were able to infer proteins’ function solely through 3D homology. Moreover, we searched through the genomes of 77 species closely related to S. cerevisiae and identified over 300 Pir homologues, with which we mapped Pir evolution throughout the last 110 million years. Finally, by comparing homologous genomic loci, we show that S. cerevisiae is the only species within the Saccharomyces clade to lose one of its Pir proteins. As such, our findings point towards the role and diversity of Pir proteins in budding yeasts, thus opening novel avenues in yeast surface engineering.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija



POVEZANOST RADA


Projekti:
IP-2019-04-2891 - Biotehnološka primjena ugradnje heterolognih proteina u stanične stijenke kvasaca (PRODIS) (Mrša, Vladimir, HRZZ - 2019-04) ( CroRIS)

Ustanove:
Prehrambeno-biotehnološki fakultet, Zagreb

Poveznice na cjeloviti tekst rada:

congress2022.hdbmb.hr

Citiraj ovu publikaciju:

Žunar, Bojan; Lozančić, Mateja; Martinić-Cezar, Tea; Teparić, Renata; Mrša, Vladimir
THE ROLE AND DIVERSITY OF PIR PROTEINS IN CELL WALLS OF BUDDING YEASTS // HDBMB22 From Science to Knowledge / Dulić, Morana ; Sinčić, Nino ; Vrhovac Madunić, Ivana (ur.).
Zagreb: Hrvatsko Društvo za Biotehnologiju, 2022. str. 165-165 (poster, nije recenziran, sažetak, znanstveni)
Žunar, B., Lozančić, M., Martinić-Cezar, T., Teparić, R. & Mrša, V. (2022) THE ROLE AND DIVERSITY OF PIR PROTEINS IN CELL WALLS OF BUDDING YEASTS. U: Dulić, M., Sinčić, N. & Vrhovac Madunić, I. (ur.)HDBMB22 From Science to Knowledge.
@article{article, author = {\v{Z}unar, Bojan and Lozan\v{c}i\'{c}, Mateja and Martini\'{c}-Cezar, Tea and Tepari\'{c}, Renata and Mr\v{s}a, Vladimir}, year = {2022}, pages = {165-165}, keywords = {Pir proteins, Alphafold2, in silico prediction of protein structures}, title = {THE ROLE AND DIVERSITY OF PIR PROTEINS IN CELL WALLS OF BUDDING YEASTS}, keyword = {Pir proteins, Alphafold2, in silico prediction of protein structures}, publisher = {Hrvatsko Dru\v{s}tvo za Biotehnologiju}, publisherplace = {Brela, Hrvatska} }
@article{article, author = {\v{Z}unar, Bojan and Lozan\v{c}i\'{c}, Mateja and Martini\'{c}-Cezar, Tea and Tepari\'{c}, Renata and Mr\v{s}a, Vladimir}, year = {2022}, pages = {165-165}, keywords = {Pir proteins, Alphafold2, in silico prediction of protein structures}, title = {THE ROLE AND DIVERSITY OF PIR PROTEINS IN CELL WALLS OF BUDDING YEASTS}, keyword = {Pir proteins, Alphafold2, in silico prediction of protein structures}, publisher = {Hrvatsko Dru\v{s}tvo za Biotehnologiju}, publisherplace = {Brela, Hrvatska} }




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