Naslov
ORTHODONTIC BRACKETS AND BANDS - A SOURCE OF HARMFUL METAL IONS?

Autori
Sabadi, Domagoj ; Dežulović, Lara ; Čanadi Jurešić, Gordana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts of the Congress of the Croatian Society of Biochemistry and Molecular Biology HDBMB22: From Science to Knowledge / Dulić, Morana ; Sinčić, Nino ; Vrhovac Madunić, Ivana - Zagreb : Hrvatsko Društvo za Biotehnologiju, 2022, 77-77

Skup
Congress of the Croatian Society of Biochemistry and Molecular Biology: From Science to Knowledge (HDBMB22)

Mjesto i datum
Brela, Hrvatska, 28.09.2022. - 01.10.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
metal ions ; orthodontic brackets, orthodontic bands, vitality ; viability ; Saccharomyces cerevisiae

Sažetak
Brackets and bands make up a major part of fixed orthodontic appliances. Nowadays, an increasing number of (young) people are starting to wear orthodontic appliances, unaware of the potential harmful effects that its components could have on their organism. The release of metal ions from metal parts of orthodontic appliances is becoming a growing concern of the orthodontic world because, in addition to contact dermatitis, acute or chronic toxicity is also possible to occur. The aim of this study was to examine the effects of ions released from brackets and bands on the vitality and viability of yeast cells. Two strains of Saccharomyces cerevisiae yeast were used: W303 and wild type. Two types of experimental solutions with released ions were prepared – in artificial saliva and YPD media, with three different leaching periods (3, 7 and 14 days). The change in the cell size and number, and type of growth, of both yeast strains, provoked by the 24 hours growth in differently prepared solutions, were measured by LUNA-FLTM (Logos, Biosystems) cell counter. Vitality was observed by the change in cell's metabolic activity using the LIVE/DEAD Yeast Viability Kit (Thermo Fisher Scientific, USA) and XTT test. Also, an intracellular oxidation determined by the amount of ROS generated in yeast, using the H2DCFDA fluorescence method was measured. Changes/damages on the surface of used brackets and bands, were measured by SEM-EDS method. Metal ions released from orthodontic brackets and bands (Cr3+, Ni2+, Mo4+, Fe3+ and even Co2+) affect the vitality and viability of both strains of the yeast Saccharomyces cerevisiae. Ions released from bands show a higher potential to disrupt the viability of cells, while the ions from brackets show a higher potential for metabolic disturbances of both yeast strains. Strain W303 is more sensitive than wild strain (prototroph). There is a good correlation between brackets and bands surface damage, released metal ions and provoked changes in vitality and viability, and intracellular oxidation in yeast cells.

Izvorni jezik
Engleski

Znanstvena područja
Dentalna medicina, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

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