Naslov
Prooxidative effects of metal ions eluted from orthodontic appliances to human cell lines

Autori
Orešić, Sunčana ; Durgo, Ksenija ; Fiket, Željka ; Čanadi Jurešić, Gordana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts of the Congress of the Croatian Society of Biochemistry and Molecular Biology HDBMB22: From Science to Knowledge / Dulić, Morana ; Sinčić, Nino ; Vrhovac Madunić, Ivana - Zagreb : Hrvatsko društvo za biokemiju i molekularnu biologiju (HDBMB), 2022, 75-75

Skup
HDBMB22: From Science to Knowledge

Mjesto i datum
Brela, Hrvatska, 28.09.2022. - 01.10.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
metal ions ; orthodontic appliances ; prooxidative effects ; arches ; brackets ; ligatures ; bands

Sažetak
Orthodontic appliances have been used for decades as a primary way of treating orthodontic problems and achieving an aesthetically pleasing look. Each appliance consists of metal parts: arches, brackets, ligatures and rings, which are connected to the teeth with a special glue. During the period of wearing, such device will cause some damage to the oral cavity and will show local aggression towards the mucosa. In addition to physical abrasion, chemical corrosion and the release of metal ions (nickel, chromium, titanium, cobalt, iron) is also stimulated. The aim of this research was to test prooxidative effects of metal ions released by appliance parts into saliva on four human cell lines: Hep G2, CAL 27, AGS and Caco2. Concentrated artificial saliva solutions (Toni-Zucchi artificial saliva, pH 4.8), in which parts of orthodontic appliances (brackets, arches, ligatures, rings and all parts together) were immersed for 3, 7 and 14 days, respectively, were used as test samples. The effect of all samples at four different concentrations (0.1 ; 0.5 ; 1.0 and 2.0) was examined on all tested cell lines. Prooxidative effects were tested using the DCFH-DA method and φX-174 RF I plasmid linearization assay. Gel Analyser was used for measurement of the band intensity. TIBCO Statistica program was use for data analysis. The elution time affects the content of metal ions of the saliva and biological effects can be measured. Metals in saliva samples caused a slight prooxidative effect on DNA - lower ratio of supercoiled and linear plasmid form compared to the negative control, but the ratio of the supercoiled/coiled DNA was higher in comparison to the positive control. The highest toxic effect of all tested samples was achieved on CAL 27 cell line. The results show that a certain amount of those effects on cells is visible, but that it is not worrying to the point of saying that orthodontic appliances are harmful for human health.

Izvorni jezik
Engleski

Znanstvena područja
Dentalna medicina, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

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