Pregled bibliografske jedinice broj: 1218643
Different conjugation strategies of gold nanoparticles with anti-SARS-CoV-2 antibodies for ultrasensitive detection of coronavirus
Different conjugation strategies of gold nanoparticles with anti-SARS-CoV-2 antibodies for ultrasensitive detection of coronavirus // Arhiv za higijenu rada i toksikologiju-SynChemNano 2022
Zagreb, Hrvatska, 2022. str. 18-18 (predavanje, domaća recenzija, sažetak, znanstveni)
CROSBI ID: 1218643 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Different conjugation strategies of gold
nanoparticles with anti-SARS-CoV-2 antibodies for
ultrasensitive detection of coronavirus
Autori
Kalčec, Nikolina ; Frkanec, Ruža ; Šišić, Marcela ; Kurtović, Tihana ; Brizić, Ilija ; Ćurlin, Marija ; Frkanec, Leo ; Horvat, Lucija ; Vinković Vrček, Ivana
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Arhiv za higijenu rada i toksikologiju-SynChemNano 2022
/ - , 2022, 18-18
Skup
Synergy at chemistry-nanotechnology interface
Mjesto i datum
Zagreb, Hrvatska, 28.05.2022
Vrsta sudjelovanja
Predavanje
Vrsta recenzije
Domaća recenzija
Ključne riječi
Gold nanoparticles, SARS-CoV-2 virus
Sažetak
In recent years, gold nanoparticles (AuNPs) have attracted vast interest in biosensing applications. Their unique functional and surface plasmon resonance properties, coupled with possibility to bound various biomolecules (e.g., antibodies, DNA, and RNA aptamers) may significantly enhance diagnostic features. Therefore, functionalizing AuNPs with antibodies could be a new approach for detecting COVID-19 caused by SARS-CoV-2 virus (1, 2). Here we present three conjugation strategies in order to assure efficient binding of monoclonal anti-SARS-CoV-2 antibodies to AuNPs in the oriented way: a) direct conjugation of antibodies by electrostatic interactions or physical adsorption on AuNPs, b) conjugation mediated by glycopeptide-functionalized AuNPs using peptidoglycan monomer, GlcNAc- MurNAc-L-Ala-D-isoGln- mesoDAP(NH2)-D-Ala-D-Ala (PGM)) and c) covalent conjugation using EDC/NHS method. The AuNPsantibodies conjugates were characterized by various spectroscopic and electrophoretic techniques. Additionally, gold nanoparticles labelled with secondary antibodies (Immunogold) were used to determine the amount of available epitopes of the primary monoclonal antibodies on the metal surface. Preliminary results showed that binding efficiency of antibody-AuNPs conjugate can be improved using the covalent binding strategy with their high potential for use in SARS-CoV-2 virus detection.
Izvorni jezik
Engleski
POVEZANOST RADA
Projekti:
HRZZ-PZS-2019-02-4323 - Siguran pristup za razvoj nano-sustava za ciljanu isporuku lijekova u mozak (SENDER) (Vinković Vrček, Ivana, HRZZ ) ( CroRIS)
Profili:
Marija Ćurlin (autor)
Marcela Šišić (autor)
Leo Frkanec (autor)
Ilija Brizić (autor)
Tihana Kurtović (autor)
Nikolina Kalčec (autor)
Ivana Vinković Vrček (autor)
Ruža Frkanec (autor)