Naslov
Transcriptomics of euchromatic satellite DNAs in embryogenesis and development

Autori
Despot-Slade, Evelin ; Volarić, Marin ; Meštrović, Nevenka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Epigenome inheritance and reprogramming in health and disease : Abstract book / - , 2022, 17-17

Skup
EMBO Workshop Epigenome inheritance and reprogramming in health and disease

Mjesto i datum
Split, Hrvatska, 22.09.2022. - 26.09.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
satellite DNA ; transcriptome ; Tribolium castaneum

Sažetak
Satellite DNAs (satDNAs) are rapidly evolving non-coding DNA, characterized by tandem monomer arrangement into long arrays. They are mostly described within (peri)centromeric heterochromatin with a role in chromosome segregation. However, recent studies disclosed that satDNAs could also be located in euchromatin. Although satDNAs comprise a substantial portion of all eukaryotic genomes, little is known about their functions. Recently it has been found that many satDNAs are transcribed and satDNA transcripts could modulate gene expression during development. The beetle Tribolium castaneum has become one of the most important models in the field of evolution and development. Combined with thoroughly researched embryogenesis and holometaboly, T. castaneum provides a unique platform for the study of satDNA transcription in the gene environment. Genome-wide analysis of T. castaneum genome revealed that in addition to high copy (peri)centromeric satDNA, long arrays of nine satDNAs (Cast1-Cast9) are located almost exclusively in euchromatin. We analyzed expression pattern of nine euchromatic satDNAs (Cast1-Cast9) using total and small RNA portions in different developmental stages. Transcriptional analysis revealed differentially expressed euchromatic satDNAs, with specific patterns observed throughout development. Further, examination of small RNAs showed preferential sequence length, indicating involvement of specific RNA subtypes. Interestingly, transcription of small satRNAs predominantly originates from the most conserved part of satDNA monomer, suggesting that the monomer locus and its associated small satRNA could have important and conserved functions. Overall, observed trends allow us to further investigate the possible functional role of satellite transcripts affecting the expression of genes in the vicinity during embryogenesis and development.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA