Naslov
Strontium/silver-co-substituted hydroxyapatite derived from biogenic source with non-cytotoxic and antibacterial properties: A multifunctional biomaterial

Autori
Ressler, Antonia ; Ivanković, Tomislav ; Polak, Bruno ; Ivanišević, Irena ; Kovačić, Marin ; Urlić, Inga ; Hussainova, Irina ; Ivanković, Hrvoje

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
32nd Symposium and Annual Meeting of the International Society for Ceramics in Medicine : book of abstracts / - , 2022, 171-171

Skup
32nd Symposium and Annual Meeting of the International Society for Ceramics in Medicine : Bioceramics 32

Mjesto i datum
Venecija, Italija, 20.09.2022. - 23.09.2022

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
antibacterial ; bone regeneration ; hydroxyapatite ; silver ; strontium

Sažetak
Hydroxyapatite (HAp) is the most widely used calcium phosphate for hard tissue applications due to its compositional similarity to biological apatite, biocompatibility and bioactivity. As bone apatite is a multi-substituted carbonated HAp containing trace elements, one of the used approaches to improve the biological and physicochemical properties of HAp is ionic substitution. To achieve a good balance between cell biomaterial interactions and anti-bacterial properties, a Sr-substituted HAp with potential regenerative properties was co-substituted with Ag+ ions to offset the cytotoxicity of higher substitution levels of Ag+ ions and stimulate osteogenic growth. A series of single-substituted HAp with Sr2+ and Ag+ ions, and Ag/Sr-co-substituted HAp with different substitution degrees (0, 1, 2.5 and 5 mol%) were obtained by wet precipitation method from cuttlefish bone in order to obtain biomimetic multi-substituted HAp. Rietveld refinement studies revealed an increase of cell volume (Å3), a-axis (Å), b-axis (Å) and c-axis (Å) for all obtained HAp powders, due to larger ionic radius of Sr2+ (1.12 Å) and Ag+ (1.15 Å) in comparison to substituted Ca2+ (0.99 Å) ion. The element mapping reflected the uniform distribution of the Ca, P, Ag and Sr elements in the precipitated powders. The zeta-potential values of the Ag/Sr-co-substituted HAp samples revealed that all the samples were negatively charged and the obtained zeta-potential values expressed a downward trend with increasing buffer pH. The antibacterial effect was evaluated by inhibition zone and spread plate analysis, while the surface of samples was observed after inhibition zone analysis by scanning electron microscopy. The antibacterial effect was confirmed for all HAp powders substituted with Ag+ ions against Gram-positive S. aureus and Gram-negative E. coli, representative bacteria in clinical bone infections. Further, the non-cytotoxicity was confirmed by using human embryonic kidney 293 and human mesenchymal stem cells.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo, Temeljne tehničke znanosti

POVEZANOST RADA