Pregled bibliografske jedinice broj: 1215133
Detection of the virulence genes of Vibrio harveyi bacteria and testing the suitability of an alternative host for studying virulence
Detection of the virulence genes of Vibrio harveyi bacteria and testing the suitability of an alternative host for studying virulence // 4th EAFP UK & Ireland EAFP branches meeting, Weymouth, UK
Weymouth, 2022. str. 14-14 (predavanje, podatak o recenziji nije dostupan, kratko priopćenje, znanstveni)
CROSBI ID: 1215133 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Detection of the virulence genes of Vibrio harveyi
bacteria and testing the suitability of an
alternative host for studying virulence
Autori
Zupičić, Ivana Giovanna ; Zrnčić, Snježana ; Desbois, Andrew ; Monaghan, Sean
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, kratko priopćenje, znanstveni
Izvornik
4th EAFP UK & Ireland EAFP branches meeting, Weymouth, UK
/ - Weymouth, 2022, 14-14
Skup
4th EAFP UK & Ireland EAFP branches meeting
Mjesto i datum
Weymouth, Ujedinjeno Kraljevstvo, 14.09.2022. - 15.09.2022
Vrsta sudjelovanja
Predavanje
Vrsta recenzije
Podatak o recenziji nije dostupan
Ključne riječi
V. harveyi, Galleria mellonella, virulence genes
Sažetak
Aquaculture plays an increasingly important role in global food production, but bacterial diseases still cause significant economic losses and negatively affect animal welfare. The bacterium V. harveyi is an emerging disease agent in global aquaculture. The virulence factors of the V. harveyi have not yet been fully elucidated. The most common (typical) virulence genes of the bacterium V. harveyi are: luxR, toxR, chiA, serine protease, vhh, vhml and vhs. A collection of 16 bacterial strains sampled from farmed sea bass (Dicentrarchus labrax) and sea bream (Sparus aurata) in different Mediterranean countries were sequenced using nanopore MinION and Illumina technology. Obtained sequences were analyzed using bioinformatical tools and a reference database for bacterial virulence factors (VFDB). However, there is a strong need for use of alternative models to study the phenotypic properties of bacterial pathogens causing diseases in farmed fish. The larva of the wax moth (Galleria mellonella) is one of the alternative models that attract a lot of attention due to the simplicity and reliability of determining infections in this insect. In this present study, we also aimed to assess whether the Galleria mellonella model is a suitable alternative host for studying the virulence of V. harveyi. Larvae were inoculated with different concentrations of bacterial suspension (CFU/ml) and supernatant respectively and incubated at 22 °C and 28 °C. Mortality was monitored for 7 days.
Izvorni jezik
Engleski
Znanstvena područja
Veterinarska medicina
