Naslov
Bacterial resistance to carbapenems in Zagreb wastewater

Autori
Udiković Kolić, Nikolina ; Puljko, Ana ; Dekić Rozman, Svjetlana ; Babić, Ivana ; Jelić, Marko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
57. hrvatski i 17. međunarodni simpozij agronoma : zbornik sažetaka = 57th Croatian and 17th International Symposium on Agriculture : book of abstracts / Majić, Ivana ; Antunović, Zvonko - Vodice : Fakultet agrobiotehničkih znanosti Sveučilišta Josipa Jurja Strossmayera u Osijeku, 2022, 76-77

Skup
57. hrvatski i 17. međunarodni simpozij agronoma

Mjesto i datum
Vodice, Hrvatska, 19.06.2022. - 24.06.2022

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
antibiotic resistance ; carbapenems ; enterobacteria ; wastewater ; sewage treatment plant

Sažetak
The increasing prevalence of enterobacteria resistant to carbapenems, antibiotics that are the last resort for treating infections caused by multidrug-resistant Gram-negative bacteria, is a worrisome problem that has reached global proportions. Sewage treatment plants can be reservoirs and sources for the spread of these bacteria in natural watercourses, as well as on land through irrigation, posing a risk to human health. The aim of this study was to isolate and characterize enterobacteria (200 isolates) showing resistance to carbapenems in treated wastewater from the central wastewater treatment plant of the city of Zagreb. Sensitivity of bacterial isolates to 3 carbapenems (ertapenem, imipenem, meropenem) was tested by the microdilution method, and most isolates were resistant to all 3 carbapenems tested. The colorimetric CarbaNP assay confirmed that carbapenem resistance was mediated by carbapenemase production in most of the isolates tested. Most isolates also exhibited a multidrug- resistant phenotype, i.e., resistance to at least 3 classes of antibiotics. PCR analysis confirmed the presence of clinically relevant genes for carbapenemases (blaKPC, blaNDM, blaVIM, blaIMP, or blaOXA-48) in most of the isolates tested. The results of this study suggest that treated municipal wastewater is a reservoir of clinically relevant enterobacteria resistant to carbapenems and that additional disinfection measures are needed to prevent their further spread within and outside the environment.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Interdisciplinarne prirodne znanosti, Biotehnologija, Interdisciplinarne biotehničke znanosti

POVEZANOST RADA