Naslov
Synthetic opioids induce oxidative stress and DNA damage in human neuroblastoma SH-SY5Y cell line

Autori
Rašić, Dubravka ; Jurič, Andreja ; Zandona, Antonio ; Tariba Lovaković, Blanka ; Pizent, Alica ; Kopjar, Nevenka ; Katalinić, Maja ; Kozina, Goran ; Lucić Vrdoljak, Ana ; Rešić, Arnes ; Brčić Karačonji, Irena

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the XVIth International Congress of Toxicology (ICT 2022) UNITING IN TOXICOLOGY / Toxicology Letters / Mally, Angela - , 2022, S221-S221

Skup
16th International Congress of Toxicology (ICT 2022)

Mjesto i datum
Maastricht, Nizozemska, 18.09.2022. - 21.09.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Opioids ; DNA instability ; cell viability ; toxicity

Sažetak
Synthetic opioids heroin, buprenorphine, and methadone bind to opioid receptors. The latter two have medical use with moderate to high addiction potential. The neurotoxic effects of opioids are often associated with oxidative stress that should be further investigated. The aim of this study was to determine the induction of oxidative stress parameters and the levels of DNA instability in human neuroblastoma SH-SY5Y cells treated with selected concentrations of three synthetic opioids during 24 hours. SH-SY5Y cells were treated with opioids at concentrations that did not decrease cell viability below 75% as follows: heroin at 0.58 mg/mL, buprenorphine at 11.69 mg/mL and methadone at 0.483 mg/mL. After the treatment, cells were prepared for further analysis according to specific method protocol. To determine the opioids toxicity, we measured biomarkers of oxidative stress [malondialdehyde (MDA), reactive oxygen species (ROS) production, glutathione (GSH) levels, and activities of antioxidant enzymes – superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT)], while primary DNA damage was determined using alkaline comet assay. Significantly increased MDA concentration compared to controls was measured only in cells treated with heroin. When compared to controls, cells treated with heroin had significantly increased SOD and GPx, cells treated with buprenorphine had significantly increased activity of SOD, GPx and CAT and cells treated with methadone had significantly decreased SOD and increased GPx and CAT. Buprenorphine and methadone decreased GSH concentration compared to control cells. Significantly increased level of DNA damage was detected only after treatment with buprenorphine. To our knowledge, this is the pioneer study that investigates outcomes of synthetic opioids in SH- SY5Y cells. Since this information was not known up to now, we find our preliminary results important for the better understanding of the toxicity profiles of tested compounds, and for design of upcoming experiments. Funding: This research was supported by the programme of cooperation between the Institute for Medical Research and Occupational Health (Zagreb, Croatia) and the University North (Varaždin, Croatia) and the Croatian Science Foundation’s grant number HrZZ-UIP-2017-05-7260.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Javno zdravstvo i zdravstvena zaštita

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