Naslov
The impact of neonicotionoid on behaviour and metabolomics of carabid beetles and its spreading by trophic interactions

Autori
Ivanković Tatalović, Lara ; Mašek, Tomislav ; Šerić Jelaska, Lucija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
20th European Carabidologist Meeting - Book of Abstracts / - , 2022, 14-14

Skup
20th European Carabidologist Meeting

Mjesto i datum
Varšava, Poljska, 25.07.2022. - 27.07.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Carabid beetles, feeding, locomotion, metabolomics, superoxide dismutase, thiamethoxam

Sažetak
Neonicotinoids are widely applied insecticides that can cause nervous stimulation, paralysis, or death of insects. Predatory carabids may serve in pest control in agriculture and forestry. Exposure to neonicotinoids can impact their behaviour and physiology, which can result in a decline in their predation efficiency. The goal of this study was to test the effect of direct, short-term exposure to neonicotinoid thiamethoxam on the feeding rate, locomotion, metabolomics, and oxidative stress level in a predatory carabid Abax parallelus and Abax ovalis, and possible transfer from prey to predators via trophic interactions. The dipping method was used to expose the beetles to increasing concentrations of thiamethoxam, after which they were offered fresh blowfly larvae and left to feed overnight. The mass of the consumed food per body weight was calculated for each beetle. For 48 hours following the treatment the locomotor ability was observed, and every beetle was classified as normal, slightly intoxicated, intoxicated, or dead/moribund. Metabolomics profile and superoxide dismutase levels in their tissue were scanned. To check for the bioaccumulation of the thiamethoxam, Abax ovalis was fed with earthworms that had been kept for seven days in the soil contaminated with the thiamethoxam (3 mg/kg, 8 mg/kg, and control group). 24 hours after feeding their body burden with thiamethoxam residues were measured using inductively coupled plasma mass spectrometry. The individuals treated with higher concentrations of thiamethoxam (20 and 40 mg AI/L) fed significantly less and had a higher share of intoxicated and moribund individuals while feeding rate and locomotion did not differ significantly between control and groups treated with lower (3.9 and 9.1 mg AI/L) concentrations. Furthermore, the concentration of metabolites such as succinate and d-glucose significantly differed between treated and the control group, which indicates a possible disruption in energy production. Thiamethoxam was detected in all carabid beetles that fed on the earthworms from the soil that was spiked with this insecticide. This research demonstrates a wide spectre of sublethal effects on the behaviour and physiology of carabid beetles following thiamethoxam intoxication, and that it can be spread by prey-predator interactions in the laboratory. Research on long-term exposure to lower doses is recommended.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Veterinarska medicina, Poljoprivreda (agronomija)

POVEZANOST RADA