Naslov
Effects of prepartal body condition on postpartal energy metabolism and paraoxonase-1 activity in dairy cows

Autori
Folnožić, Ivan ; Đuričić, Dražen ; Samardžija, Marko ; Vince, Silvijo ; Beer Ljubić, Blanka ; Turk, Romana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
World Buiatrics Congress, September 4th-8th, Madrid 2022 Abstract Book / - , 2022, 198-199

Skup
World Buiatrics Congress, September 4th-8th, Madrid 2022

Mjesto i datum
Madrid, Španjolska, 04.09.2022. - 08.09.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Dairy cows, body condition, negative energy balance, oxidative stress, lipid mobilization
(Dairy cows, body condition, negative energy balance, oxidative stress, lipid mobilizatio)

Sažetak
Objective: In early lactation, energy availability from dry matter intake is often insufficient to meet energy requirements of dairy cows. Overconditioned dairy cows usually mobilize more lipids from adipose tissue than cows with optimal body condition resulting in increased ketone compound formation and possibly development of subclinical and clinical ketosis. Additionally, lipid mobilisation is accompanied with increased formation of reactive oxygen species (ROS) and oxidative stress development. Thus, the aim of the study was to evaluate glucose, nonesterified fatty acids (NEFA) and β-hydroxybutyrate (BHB) concentrations and antioxidant paraoxonase-1 (PON1) activity in overconditioned cows and those with optimal body condition. Material and Methods: The study included twenty- four Holstein dairy cows assigned into two groups according to body condition 30 days before parturition: (1) the optimal cows (n=12) that comprised cows with optimal body condition score (BCS) between 3.25-3.75, and the adipose group (n=12) that comprised overconditioned cows with BCS ≥ 4. Blood samples were taken on days -30, -10, -2, 0, 5, 12, 19, 26 relative to parturition. Glucose concentration was measured by the commercial kit reagent (Beckman Coulter Biomedical Ltd., Ireland) on the biochemical analyser Beckman Coulter AU 680 (Beckman Coulter Biomedical Ltd., Ireland). Serum BHB and NEFA were analysed by the commercial kits (Randox, Ireland) on the biochemical analyser Beckman Coulter AU 640 (Beckman Coulter Biomedical Ltd., Ireland). The PON1 activity was measured by the spectrophotometric assay using paraoxon as a substrate on Beckman Coulter AU 680. Statistical analysis was performed by SAS 9.3 Software. Results: Adipose cows had significantly higher (P<0.05) glucose concentration at the day of parturition (4.66 mmol/L) than optimal cows (3.11 mmol/L). However, in early lactation, glucose level was significantly lower (P<0.05) in adipose cows on days 12 and 19 (2.33 and 2.46 mmol/L, respectively) compared to cows with optimal condition at the same sampling points (3.21 and 3.18 mmol/L, respectively). Serum NEFA was significantly increased in both optimal and overconditioned cows on day of parturition (1.01 and 0.80 mmol/L, respectively) and 5 days of lactation (0.93 and 0.88 mmol/L, respectively) compared to values before parturition. Serum BHB was significantly higher (P<0.05) in overconditioned cows (2.3 mmol/L) compared to cows with optimal condition (1.8 mmol/L) considering all samples in each group. There were no significant differences (P>0.05) in NEFA and BHB concentrations between the two groups at each sampling points. Nevertheless, even not statistically significant, BHB level increased in adipose cows after parturition on day 12 and 19 (3.00 and 3.26 mmol/L, respectively) indicating subclinical and clinical ketosis. In optimal cows, BHB level was the highest 5 days after calving (2.25 mmol/L) suggesting subclinical ketosis. Serum PON1 activity was higher in adipose cows 2 days before parturition and lower 5 days of lactation compared to the optimal cows, even without statistical significance. Conclusion: Transition period in dairy cows is accompanied with negative energy balance resulting in lipid mobilisation indicated by increased NEFA concentration in blood and decreased antioxidant status. Overconditioned dairy cows had lower glucose availability in early lactation and thus produced more ketone compounds to satisfy energy requirements of the body being susceptible to subclinical and clinical ketosis.

Izvorni jezik
Engleski

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