Naslov
Genetic characterization of piroplasms infecting cervids in Croatia

Autori
Pintur, Krunoslav ; Dumić, Tomislav ; Beck, Relja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
10th International Deer Biology Congress Book of Abstracts / Konjević, Dean ; Bujanić, Miljenko ; Škvorc, Nikolina - Osijek : Veteriranrski fakultet Sveučilišta u Zagrebu, 2022, 139-140

ISBN
978-953-8006-39-5

Skup
10th International Deer Biology Congress

Mjesto i datum
Osijek, Hrvatska, 04.09.2022. - 09.09.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
piroplasma, Babesia, Theileria, cervids, 18rRNA, PCR, phylogenetic analysis, Croatia

Sažetak
Context: The piroplasma are obligate intracellular apicomplexan protozoan parasites transmitted by the ticks. Many species of the genera Babesia and Theileria parasitize domestic and wild mammals. Aims: To investigate prevalence and species of Babesia and Theileria in Cervids in Croatia.species using molecular approach. Methods: Cervid spleen samples were collected after the regular cull in 2008 and 2009 on three locations in Croatia ; Gorski Kotar, Moslavačka gora and Baranja. Spleen of 164 animals was sampled (50 grams): 49 roe deer (Capreolus capreolus), 102 red deer (Cervus elaphus) and 13 fallow deer (Dama dama). After extracting the DNA, a targeted sequence of a small subunit 18S rRNA was multiplied using the polymerase chain reaction. The purified PCR product was sequenced, after which the nucleotide sequence comparison and phylogenetic analysis were conducted. The specific gene segment 18S rRNA was registered in 111 individuals (67.68%). Key results: Using nucleotide sequence analysis the following species or genotypes were deconfirmed: B. crassa, Babesia sp., B. divergens/capreoli, Babesia venatorum, Theileria ovis and Theileria capreoli. The highest number of positive cervids (96.08%) originated from the Moslavina region. The greatest diversity of species was found in Gorski Kotar region, where Babesia sp., B. divergens/capreoli, Babesia venatorum, T. ovis, Theileria capreoli were registered. In the Baranja region, only Theileria capreoli was proven. The incidence of piroplasma in red deer was 56.86%, in which the incidence of Babesia was 3.92% (Babesia sp. 2.94%, B.divergens/capreoli 0.98%) and Theileria capreoli was 52.94%. All sampled fallow deer were positive for Theileria capreoli. 22.45% of roe deer were positive for Babesia (18.36% cases were B. divergens/capreoli, 2.04% B. crassa and 2.04% zoonotic type of Babesia venatorum), and 59.18% on Theileria of which in. Theileria capreoli was proved in 57.14% of roe deer and T. ovis in only one roe deer sample (2.04%). Phylogenetic analysis of nucleotide sequences proved in this study and compared with the referent nucleotide sequence of gene groups that are available in gene bank (GenBank) has shown the classification in genetic groups and subgroups. Conclusion: Cervids play a significant role in the life cycle of piroplasmas with higher prevalence of Theileria than Babeisa species. It is worthy to notice detection of zoonotic species Babesia venatorum, B. crassa and potentially zoonotic isolate B.divergens/capreoli in red deer and roe deer.

Izvorni jezik
Engleski

Znanstvena područja
Interdisciplinarne prirodne znanosti, Biotehnologija, Interdisciplinarne biotehničke znanosti, Integrativna bioetika (prirodne, tehničke, biomedicina i zdravstvo, biotehničke, društvene, humanističke znanosti), Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

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