Naslov
Differences in immunoglobulin G glycosylation between influenza and COVID-19 patients

Autori
Kljaković-Gašpić Batinjan, Marina ; Petrović, Tea ; Vučković, Frano ; Hadžibegović, Irzal ; Radovani, Barbara ; Jurin, Ivana ; Đerek, Lovorka ; Huljev, Eva ; Markotić, Alemka ; Lukšić, Ivica ; Trbojević-Akmačić, Irena ; Lauc, Gordan ; Gudelj, Ivan ; Čivljak, Rok

Vrsta, podvrsta
Radovi u časopisima, znanstveni

Izvornik
Engineering (2022)

Status rada
Online first

Ključne riječi
influenza ; COVID-19 ; viral infection ; glycosylation ; immunoglobulin G ; pneumonia

Sažetak
The essential role of immunoglobulin G (IgG) in immune system regulation and combatting infectious diseases cannot be fully recognized without an understanding of the changes in its N-glycans attached to the asparagine 297 of the Fc domain that occur under such circumstances. These glycans impact the antibody stability, half-life, secretion, immunogenicity, and effector functions. Therefore, in this study, we analyzed and compared the total IgG glycome—at the level of individual glycan structures and derived glycosylation traits (sialylation, galactosylation, fucosylation, and bisecting N-acetylglucosamine (GlcNAc))—of 64 patients with influenza, 77 patients with coronavirus disease 2019 (COVID- 19), and 56 healthy controls. Our study revealed a significant decrease in IgG galactosylation, sialylation, and bisecting GlcNAc (where the latter shows the most significant decrease) in deceased COVID-19 patients, whereas IgG fucosylation was increased. On the other hand, IgG galactosylation remained stable in influenza patients and COVID-19 survivors. IgG glycosylation in influenza patients was more time-dependent: In the first seven days of the disease, sialylation increased and fucosylation and bisecting GlcNAc decreased ; in the next 21 days, sialylation decreased and fucosylation increased (while bisecting GlcNAc remained stable). The similarity of IgG glycosylation changes in COVID-19 survivors and influenza patients may be the consequence of an adequate immune response to enveloped viruses, while the observed changes in deceased COVID-19 patients may indicate its deviation.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Interdisciplinarne prirodne znanosti

POVEZANOST RADA