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Pregled bibliografske jedinice broj: 1211861

Unveiling the Native Morphology of Extracellular Vesicles from Human Cerebrospinal Fluid by Atomic Force and Cryogenic Electron Microscopy


Kurtjak, Mario; Kereïche, Sami; Klepac, Damir; Križan, Hrvoje; Perčić, Marko; Krušić Alić, Vedrana; Lavrin, Teja; Lenassi, Metka; Wechtersbach, Karmen; Kojc, Nika et al.
Unveiling the Native Morphology of Extracellular Vesicles from Human Cerebrospinal Fluid by Atomic Force and Cryogenic Electron Microscopy // Biomedicines, 10 (2022), 6; 1251, 10 doi:10.3390/biomedicines10061251 (međunarodna recenzija, članak, znanstveni)


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Naslov
Unveiling the Native Morphology of Extracellular Vesicles from Human Cerebrospinal Fluid by Atomic Force and Cryogenic Electron Microscopy

Autori
Kurtjak, Mario ; Kereïche, Sami ; Klepac, Damir ; Križan, Hrvoje ; Perčić, Marko ; Krušić Alić, Vedrana ; Lavrin, Teja ; Lenassi, Metka ; Wechtersbach, Karmen ; Kojc, Nika ; Vukomanović, Marija ; Zrna, Siniša ; Biberić, Maša ; Domitrović, Robert ; Grabušić, Kristina ; Malenica, Mladenka

Izvornik
Biomedicines (2227-9059) 10 (2022), 6; 1251, 10

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
extracellular vesicles ; cerebrospinal fluid ; size-exclusion chromatography ; atomic force microscopy ; cryogenic transmission electron microscopy ; morphology 1. Introduction

Sažetak
Extracellular vesicles (EVs) are membranous structures in biofluids with enormous diagnostic/prognostic potential for application in liquid biopsies. Any such downstream application requires a detailed characterization of EV concentration, size and morphology. This study aimed to observe the native morphology of EVs in human cerebrospinal fluid after traumatic brain injury. Therefore, they were separated by gravity- driven size-exclusion chromatography (SEC) and investigated by atomic force microscopy (AFM) in liquid and cryogenic transmission electron microscopy (cryo-TEM). The enrichment of EVs in early SEC fractions was confirmed by immunoblot for transmembrane proteins CD9 and CD81. These fractions were then pooled, and the concentration and particle size distribution were determined by Tunable Resistive Pulse Sensing (around 1010 particles/mL, mode 100 nm) and Nanoparticle Tracking Analysis (around 109 particles/mL, mode 150 nm). Liquid AFM and cryo-TEM investigations showed mode sizes of about 60 and 90 nm, respectively, and various morphology features. AFM revealed round, concave, multilobed EV structures ; and cryo-TEM identified single, double and multi- membrane EVs. By combining AFM for the surface morphology investigation and cryo-TEM for internal structure differentiation, EV morphological subpopulations in cerebrospinal fluid could be identified. These subpopulations should be further investigated because they could have different biological functions.

Izvorni jezik
Engleski



POVEZANOST RADA


Projekti:
--uniri-biomed-18-279 - Uspostava protokola na Sveučilištu u Rijeci za primjenu nanotehnoloških metoda: mikroskopije atomskih sila i skenirajućeg elektronskog mikroskopa prilikom vizualizacije i karakterizacije egzosoma (Malenica Staver, Mladenka; Malenica Staver, Mladenka) ( CroRIS)
HRZZ-IP-2019-04-1511 - Identifikacija cirkulirajućih biomarkera neurološkog oporavka u bolesnika s ozljedom mozga (CiMozak / CirBrain) (Grabušić, Kristina, HRZZ - 2019-04) ( CroRIS)

Poveznice na cjeloviti tekst rada:

Pristup cjelovitom tekstu rada doi www.mdpi.com

Citiraj ovu publikaciju:

Kurtjak, Mario; Kereïche, Sami; Klepac, Damir; Križan, Hrvoje; Perčić, Marko; Krušić Alić, Vedrana; Lavrin, Teja; Lenassi, Metka; Wechtersbach, Karmen; Kojc, Nika et al.
Unveiling the Native Morphology of Extracellular Vesicles from Human Cerebrospinal Fluid by Atomic Force and Cryogenic Electron Microscopy // Biomedicines, 10 (2022), 6; 1251, 10 doi:10.3390/biomedicines10061251 (međunarodna recenzija, članak, znanstveni)
Kurtjak, M., Kereïche, S., Klepac, D., Križan, H., Perčić, M., Krušić Alić, V., Lavrin, T., Lenassi, M., Wechtersbach, K. & Kojc, N. (2022) Unveiling the Native Morphology of Extracellular Vesicles from Human Cerebrospinal Fluid by Atomic Force and Cryogenic Electron Microscopy. Biomedicines, 10 (6), 1251, 10 doi:10.3390/biomedicines10061251.
@article{article, author = {Kurtjak, Mario and Kere\"{\i}che, Sami and Klepac, Damir and Kri\v{z}an, Hrvoje and Per\v{c}i\'{c}, Marko and Kru\v{s}i\'{c} Ali\'{c}, Vedrana and Lavrin, Teja and Lenassi, Metka and Wechtersbach, Karmen and Kojc, Nika and Vukomanovi\'{c}, Marija and Zrna, Sini\v{s}a and Biberi\'{c}, Ma\v{s}a and Domitrovi\'{c}, Robert and Grabu\v{s}i\'{c}, Kristina and Malenica, Mladenka}, year = {2022}, pages = {10}, DOI = {10.3390/biomedicines10061251}, chapter = {1251}, keywords = {extracellular vesicles, cerebrospinal fluid, size-exclusion chromatography, atomic force microscopy, cryogenic transmission electron microscopy, morphology 1. Introduction}, journal = {Biomedicines}, doi = {10.3390/biomedicines10061251}, volume = {10}, number = {6}, issn = {2227-9059}, title = {Unveiling the Native Morphology of Extracellular Vesicles from Human Cerebrospinal Fluid by Atomic Force and Cryogenic Electron Microscopy}, keyword = {extracellular vesicles, cerebrospinal fluid, size-exclusion chromatography, atomic force microscopy, cryogenic transmission electron microscopy, morphology 1. Introduction}, chapternumber = {1251} }
@article{article, author = {Kurtjak, Mario and Kere\"{\i}che, Sami and Klepac, Damir and Kri\v{z}an, Hrvoje and Per\v{c}i\'{c}, Marko and Kru\v{s}i\'{c} Ali\'{c}, Vedrana and Lavrin, Teja and Lenassi, Metka and Wechtersbach, Karmen and Kojc, Nika and Vukomanovi\'{c}, Marija and Zrna, Sini\v{s}a and Biberi\'{c}, Ma\v{s}a and Domitrovi\'{c}, Robert and Grabu\v{s}i\'{c}, Kristina and Malenica, Mladenka}, year = {2022}, pages = {10}, DOI = {10.3390/biomedicines10061251}, chapter = {1251}, keywords = {extracellular vesicles, cerebrospinal fluid, size-exclusion chromatography, atomic force microscopy, cryogenic transmission electron microscopy, morphology 1. Introduction}, journal = {Biomedicines}, doi = {10.3390/biomedicines10061251}, volume = {10}, number = {6}, issn = {2227-9059}, title = {Unveiling the Native Morphology of Extracellular Vesicles from Human Cerebrospinal Fluid by Atomic Force and Cryogenic Electron Microscopy}, keyword = {extracellular vesicles, cerebrospinal fluid, size-exclusion chromatography, atomic force microscopy, cryogenic transmission electron microscopy, morphology 1. Introduction}, chapternumber = {1251} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


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