Length-dependent poleward flux of sister kinetochore fibers promotes chromosome alignment

Risteski, Patrik; Božan, Domagoj; Jagrić, Mihaela; Bosilj, Agneza; Pavin, Nenad; Tolić, Iva M.

doi:10.1016/j.celrep.2022.111169

Pregled bibliografske jedinice broj: 1211787

Length-dependent poleward flux of sister kinetochore fibers promotes chromosome alignment

Risteski, Patrik; Božan, Domagoj; Jagrić, Mihaela; Bosilj, Agneza; Pavin, Nenad; Tolić, Iva M.

Length-dependent poleward flux of sister kinetochore fibers promotes chromosome alignment // Cell Reports, 40 (2022), 5; 111169, 14 doi:10.1016/j.celrep.2022.111169 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 1211787 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Length-dependent poleward flux of sister kinetochore fibers promotes chromosome alignment

Autori
Risteski, Patrik ; Božan, Domagoj ; Jagrić, Mihaela ; Bosilj, Agneza ; Pavin, Nenad ; Tolić, Iva M.

Izvornik
Cell Reports (2211-1247) 40 (2022), 5; 111169, 14

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
chromosome alignment ; mitotic spindle ; kinetochore ; microtubule poleward flux ; speckle microscopy ; mathematical model

Sažetak
Chromosome alignment at the spindle equator promotes proper chromosome segregation and depends on pulling forces exerted at kinetochore fiber tips together with polar ejection forces. However, kinetochore fibers are also subjected to forces driving their poleward flux. Here we introduce a flux-driven centering model that relies on flux generated by forces within the overlaps of bridging and kinetochore fibers. This centering mechanism works so that the longer kinetochore fiber fluxes faster than the shorter one, moving the kinetochores toward the center. We develop speckle microscopy in human spindles and confirm the key prediction that kinetochore fiber flux is length dependent. Kinetochores are better centered when overlaps are shorter and the kinetochore fiber flux slower than the bridging fiber flux. We identify Kif18A and Kif4A as overlap and flux regulators and NuMA as a fiber coupler. Thus, length-dependent sliding forces exerted by the bridging fiber onto kinetochore fibers support chromosome alignment.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Biologija, Interdisciplinarne prirodne znanosti

POVEZANOST RADA

Projekti:
EK-855158 - Molekularno podrijetlo aneuploidija u zdravim i bolesnim ljudskim tkivima (ANEUPLOIDY) (Tolić, Iva; Pavin, Nenad, EK ) ( CroRIS)
EK-647077 - Nova klasa mikrotubula u vretenu koja djeluje silama na kinetohore (NewSpindleForce) (Tolić, Iva, EK ) ( CroRIS)
HRZZ-PZS-2019-02-7653 - Mehanizmi nastajanja snopova mikrotubula potrebni za sazrijevanje diobenog vretena (SpindleForm) (Tolić, Iva, HRZZ - 2019-02) ( CroRIS)
HRZZ-IP-2019-04-5967 - Ravnoteža sila i momenta sila u diobenom vretenu (SpindleForceBalance) (Pavin, Nenad, HRZZ - 2019-04) ( CroRIS)
EK-EFRR-KK.01.1.1.04.0057 - Inovativni protokoli mikroskopije za interdisciplinarna istraživanja u biomedicini (IPSted) (Tolić, Iva Marija, EK - KK.01.1.1.04) ( CroRIS)
--KK.01.1.1.01.0004 - Provedba vrhunskih istraživanja u sklopu Znanstvenog centra izvrsnosti za kvantne i kompleksne sustave te reprezentacije Liejevih algebri (QuantiXLie) (Buljan, Hrvoje; Pandžić, Pavle) ( CroRIS)