Naslov
Lipid environment is essential for optimal activity of plasma membrane Ca2+ atpase in murine brain tissue
(Lipid environment is essential for optimal activity of plasma membrane Ca2+ atpase in murine brain tissue)

Autori
Stojanović, Mario ; Puljko, Borna ; Ilić, Katarina ; Maček Hrvat, Nikolina ; Mlinac Jerković, Kristina ; Kalanj Bognar, Svjetlana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
E-book of abstracts / - , 2022, 3255-3255

Skup
13th FENS Forum of Neuroscience

Mjesto i datum
Pariz, Francuska, 09.07.2022. - 13.07.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
gangliosides ; glycosphingolipids ; PMCA

Sažetak
Plasma membrane calcium ATPase (PMCA) is an integral resident across eukaryotic membrane systems, implicated in miscellaneous brain functions such as equilibrating cellular Ca2+ levels and signal transduction. Our preliminary data indicate that Toll-like receptor 2 deficient mice (TLR2D) exhibit differential expression of PMCA isoforms across nervous tissue. Due to firm evidence that P-type ATPases generally require a specialized lipid environment to manage assigned functions, we aimed to track specific PMCA activity and its submembrane distribution in TLR2D compared to wild-type (WT) mice. For that purpose, we used cortical, hippocampal, and cerebellar samples dissected from the TRL2D and WT brains. PMCA activity was measured by spectrophotometry, while PMCA positioning within membranes was investigated in isolated lipid raft and non-raft fractions. The results strongly imply probable interactions between PMCA and membrane lipids. The subfractionation of the membrane gave us a clue that PMCA is shifting through different lipid microenvironments with specific membrane properties. Secondly, the measured variations in the specific PMCA activity between TLR2 KO and the control animal group reflect the fact that P-type ATPases activity correlates with a different lipid-residing environment characterized by different membrane properties. These findings argue that specific PMCA arrangements in a lipid environment fine-tune differential expression of PMCA, thus possibly changing Ca2+ signaling and influencing physiological functions of the nervous system in TLR2D mice. Further study will help us understand the exact role and neurophysiological outcome of the interplay between PMCA isoforms and its neighboring membrane lipids in mammalian brain tissue.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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