Naslov
The Effect of Ascorbic Acid Treatment on Osteosarcoma Stem Cells Grown in the presence and absence of FGF2

Autori
Caput Mihalic, Katarina ; Ledinski, Maja ; Pusic, Maja ; Antunovic, Maja ; Librenjak, Lucija ; Simic Jovicic, Marijana ; Panek, Marina ; Urlic, Inga

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Innovative Cancer Science: Translating Biology to Medicine- EACR2022 / - Sevilla, 2022, 503-503

Skup
28th Congress of the European Association for Cancer Research (EACR 2022 Congress) "Innovative Cancer Science: Translating Biology to Medicine"

Mjesto i datum
Sevilla, Španjolska, 20.06.2022. - 23.06.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Osteosarcoma Stem Cells, Ascorbic Acid, FGF2

Sažetak
Introduction Osteosarcoma stem cell (Os-CSC) are mesenchymal origin stem cells derived from patient biopsies. The selection of stem cell population is achieved by spheroid growth in restrictive conditions through two generations. The essential component for their growth is the addition of fibroblast growth factor 2 (FGF2) that acts by blocking differentiation and promoting proliferation. Material and Methods The purpose of this study is to erradicate osteosarcoma stem cell population responsible for disease recurrence. According to previous in vitro studies, ascorbic acid (AA) can induce apoptosis of Os-CSC. However, the mechanisam of action is still unclear. AA could induce differentiation of Os-CSC and collagen synthesis as well as it can interact with metabolism. As FGF2 is the differentiation blocker, we wanted to test the effect of AA with and without FGF2. Therefore, we performed MTT assay and calculated IC50 values. Gene expression analysis for stemness and differentiation markers was also performed. Bioenergetic profiling of examined cell lines was performed using Seahorse XF Cell Energy Phenotype Test Kit. Metabolic changes were confirmed by Western analysis of GAPDH level. Results and Discussions The IC50 value for HEK, U2OS and hMSC cells ranged from 15.26 mM to 25.63 mM AA, while the IC50 value for Os-CSC ranged from 3.5 mM to 10.5 mM AA without FGF2 and 5.5-11 mM with FGF2. The presence of FGF2 blocked the collagen and alkaline phosphatase expression. However, the with AA restored AP expression. FGF2 did not affect basal metabolism of Os-CSC, while AA treatment increased it. Other cells showed slightly higher basal metabolic activity. Stressors increased OCAR while there was a slight increase ECAR in Os-CSC. Os-CSC have more quiescent metabolism, which is activated by treatment with AA. The complete depletion of GAPDH expression in Os-CSC treated with AA in the abscence of FGF2 was observed. Conclusion This study examined the effect of AA on Os-CSC isolated from tumour tissue samples and grown in the presence and abscence of the FGF2. The presence of FGF2 makes Os-CSC less sensitive to AA treatment. FGF2 does interfere with collagen synthesis as well as metabolic phenotype so it is important to elucidate further the interference with AA.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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