Naslov
Determination of quinolones, macrolides, sulfonamides and tetracyclines in honey- QUECHERS sample preparation and UHPLC-MS/MS analysis

Autori
Varenina, Ivana ; Bilandžić, Nina ; Varga, Ines ; Solomun Kolanović, Božica ; Božić Luburić, Đurđica

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
Proceedings of the EuroResidue IX conference / Van Ginkel, Leendert A. - Wageningen : Wageningen UR, 2022, 503-509

ISBN
9789464374940

Skup
EuroResidue IX conference

Mjesto i datum
Sint-Michielsgestel, Nizozemska, 22.05.2022. - 25.05.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
antibiotics ; honey ; QUECHERS ; UHPLC-MS/MS ; food safety

Sažetak
Monitoring the health of the beehive and timely prevention of any possible infection with various bacteria, mould, viruses or parasites is exceptionally important in beekeeping. Antibiotics may be found in honey originating from the environment and resulting from improper beekeeping practices. Enabling the detection of antibiotic residues in honey and suppressing the development of antibiotic resistance, require the development of a sensitive multi-class method for the determination of antibiotics. For the purpose of analysing honey, a screening and confirmatory method for the determination of 36 antibiotics was developed. The QUECHERS procedure and ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was selected to achieve high sensitivity and selectivity. Validation according to the new Commission Implementing Regulation (EU) 2021/808 included following performance characteristics: selectivity, trueness, precision, decision limit (CCα), detection capability (CCβ) and relative matrix effect. The method was validated in the measurement range from 1 to 20 μg/kg, where maximum trueness and acceptable coefficients of variation were achieved. For unauthorized pharmacologically active substances, the CCα was determined, ranging from 0.16 μg/kg for sulfaquinoxaline to 3.67 μg/kg for difloxacin. The high matrix influence of floral and chestnut honey indicated the need for quantitative analysis by using the matrix calibration curve.

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita

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