Pregled bibliografske jedinice broj: 1209563
Simultaneous staining of endoplasmic reticulum and lipid droplets by naphthol-aminonaphthalimide conjugates and photoinduced antiproliferative effects
Simultaneous staining of endoplasmic reticulum and lipid droplets by naphthol-aminonaphthalimide conjugates and photoinduced antiproliferative effects // Dyes and pigments, 206 (2022), 110651, 15 doi:10.1016/j.dyepig.2022.110651 (međunarodna recenzija, članak, znanstveni)
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Naslov
Simultaneous staining of endoplasmic reticulum and
lipid droplets by naphthol-aminonaphthalimide
conjugates and photoinduced antiproliferative
effects
Autori
Sambol, Matija ; Košćak, Marta ; Uzelac, Lidija ; Kralj, Marijeta ; Piantanida, Ivo ; Basarić, Nikola
Izvornik
Dyes and pigments (0143-7208) 206
(2022);
110651, 15
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
Naphthols ; Quinone methides ; Aminonaphthalimides ; Fluorescence labeling FRET ; Photo-induced antiproliferative activity
Sažetak
A detailed study of photophysical properties and photochemical reactivity of five novel naphthol- aminonaphthalimide conjugates 1a-1e (differing in the linker length between two chromophores) showed efficient Förster resonance energy transfer (FRET) from naphthol to aminonaphtalimide, preventing the photodehydration reaction to yield quinone methides (QM). Nevertheless, 1a and 1e showed photoinduced bioreactivity. Derivatives 1a and 1e showed moderate affinity toward both, ds-DNA/RNA and serum albumin (BSA), yielding opposite emission response ; strong quenching for DNA/RNA and strong increase with remarkable blue shift for BSA. Confocal fluorescent microscopy and collocalisation experiments performed on human cells lines showed efficient cellular uptake with no apparent toxicity and localization of the dyes in intracellular lipidous membranes, mostly endoplasmic reticulum (ER) and lipid droplets (LD). Owing to different fluorescent response in these organelles due to different polarity, compounds 1a-1e have potential for simultaneous staining of ER and lipid droplets. The irradiation at 350 nm of compound-treated cells resulted in very strong cytotoxic effect, evident at micromolar concentration. Thus, presented dyes are promising lead compounds to novel photo-induced theranostic agents.
Izvorni jezik
Engleski
Znanstvena područja
Kemija
POVEZANOST RADA
Projekti:
--IP-2019-04-8008 - Fotokemija: Reakcijski mehanizmi i primjene u organskoj sintezi i biologiji (PhotoApp) (Basarić, Nikola) ( CroRIS)
IP-2014-09-6312 - Supramolekulska kontrola fotokemijskih reakcija eliminacije (SupraPhotoE) (Basarić, Nikola, HRZZ - 2014-09) ( CroRIS)
HRZZ-IP-2018-01-5475 - Višekromoforne probe za prepoznavanje pojedinih struktura DNA, RNA i proteina (BioMultiChromoProbes) (Piantanida, Ivo, HRZZ - 2018-01) ( CroRIS)
Ustanove:
Institut "Ruđer Bošković", Zagreb
Profili:
Nikola Basarić
(autor)
Ivo Piantanida
(autor)
Lidija Uzelac
(autor)
Marijeta Kralj
(autor)
Marta Jurković
(autor)
Matija Sambol
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus