Naslov
HLA class II alleles and haplotypes in type 1 diabetes mellitus and type 1 diabetes mellitus associated with autoimmune comorbidities in Croatian patients: A single center study

Autori
Marczi, Saška ; Suver Stević, Mirjana ; Bačun, Tatjana ; Marušić, Romana ; Bugrin, Anđelka ; Lekić, Ivan ; Samardžija, Marina

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
HLA Immune Response Genetics / - , 2022, 538-539

Skup
36th European Immunogenetics and Histocompatibility Conference

Mjesto i datum
Amsterdam, Nizozemska, 17.05.2022. - 20.05.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Human leukocyte antigen ; Type 1 diabetes mellitus ; Autoimmune thyreoiditis ; Celiac disease

Sažetak
The aim of this study was to investigate the distribution of HLA-DRB1, HLA-DQA1 and HLA-DQB1 alleles and haplotypes in a group of East Croatian patients with type 1 diabetes (T1D) and T1D associated with autoimmune thyroiditis (AITD) and celiac disease (CD). Additionally, to determine HLA alleles and haplotypes which contribute to susceptibility/protection to/against analyzed diseases. This retrospective study included 120 TIDM patients (59 male, 61 female, mean age of 9+/-4.89 years), 79 of them (65.8%) were T1DM patients without associated autoimmune disorders, 33 (27, 5%) had T1DM associated with AITD and 8 (6.7%) had T1DM associated with CD. The control group consisted of 120 healthy, unrelated, blood donor volunteers from East Croatia. Low to medium HLA-DRB1, -DQA1 and - DQB1 typing was performed in the period of 2009-2020 using PCR- SSP and PCR-SSO techniques. Comparison was conducted using Fisher's exact test and odds ratio (95% CI). In comparison to controls, the frequencies of HLA-DRB1*03, -DRB1*04, -DQB1*02 and DQB1*03:02 were significantly higher in T1DM (OR 6.03, OR 5.24, OR 3.64 and OR 4.65, respectively, p < 0.0001), in T1DM-AITD (OR 3.82, p = 0.0005 ; OR 6.82, p < 0.0001 ; OR 2.58, p = 0.0039 and OR 7.35, p < 0.0001, respectively) and in TIDM-CD group (OR 7.16, p = 0.0006 ; OR 4.25, p = 0.0223 ; OR 2.69, p = 0.0083 and OR 7.24, p = 0.0006, respectively). The HLA-DQA1*03 was significantly more represented in T1DM and T1DM-AITD while HLA-DQA1*05 was more represented in T1DM and T1DM-CD groups. HLA-DRB1*03-DQA1 05-DQB1*02 and HLA-DRB1*04-DQA1*03-DQB1*03:02 haplotypes were significantly associated with T1DM (OR 4.81, p < 0.0001 and OR 8.30, p < 0.0001, respectively), T1DM-AITD (OR 3.18, p = 0.0035 and OR 12.37, p < 0.0001) and T1DM-CD (OR 5.72, p = 0.0028 and OR 8.44, p = 0.0027, respectively). More differences were observed in protective HLA profiles between the analyzed groups compared to HLA profiles associated with predisposition to T1DM and T1DM associated with AITD and CD.

Izvorni jezik
Engleski

Znanstvena područja
Interdisciplinarne prirodne znanosti, Temeljne medicinske znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA