Pregled bibliografske jedinice broj: 1209121
A screening of an organophosphate’s effect on the plasma membrane organization of the mouse brain
A screening of an organophosphate’s effect on the plasma membrane organization of the mouse brain // FEBS Open Bio, 12 (2022), Suppl 1
Lisabon, Portugal, 2022. str. 255-256 doi:10.1002/2211-5463.13440 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 1209121 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
A screening of an organophosphate’s effect on the
plasma membrane organization of the mouse brain
Autori
Maček Hrvat, Nikolina ; Ilić, Katarina ; Puljko, Borna ; Mlinac Jerković, Kristina, Stojanović, Mario ; Kalanj Bognar, Svjetlana ; Kovarik, Zrinka
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
FEBS Open Bio, 12 (2022), Suppl 1
/ - , 2022, 255-256
Skup
The Biochemistry Global Summit
Mjesto i datum
Lisabon, Portugal, 06.07.2022. - 14.07.2022
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
organophosphate ; plasma membrane ; lipid raft ; ganglioside GM1 ; flotillin-1 ; transferrin receptor
Sažetak
Organophosphates (OPs) such as nerve agents are lipophilic molecules that inhibit the enzyme acetylcholinesterase (AChE), which has an essential role in hydrolysing the neurotransmitter acetylcholine. The inhibition of AChE leads to the onset of lifethreatening symptoms induced by the overstimulation of nicotinic and muscarinic receptors, which can result in death. Oxime compounds are used for reactivation of inhibited AChE, but there is no universal therapy for exposure to different OPs. Moreover, the effect of the OPs is not limited to cholinergic functions but affects neuritogenesis, cellcell interactions, apoptosis, synaptogenesis, amyloid fibre formation, and they possess membrane disrupting potential due to their lipophilicity. The compartmentalization of the plasma membrane into lipid rafts has an important role in cellular signalling, endocytosis, exocytosis, and other cellular processes. Lipid raft (LR) disruption can lead to apoptosis, disorders in regulation of neurotransmission and protein trafficking, and is associated with several diseases. Herein, we investigated whether an OP induces the reorganization of the membrane subdomains. Mice exposed to a sublethal dose of nerve agent were compared to mice treated with an oxime 30 minutes after OP and both groups compared to control mice without any treatment. Mice were sacrificed 1.5h, 2 and 4 days after OP administration, brains were dissected and snapfrozen. LR were isolated from brain cortices by ultracentrifugation in discontinuous sucrose gradients and Western blotting was performed for LR markers ganglioside GM1 and Flotillin1, and nonlipid raft marker transferrin receptor. The results showed that there were slight disturbances in the distribution of the markers with regard to the OP exposure and therapy when compared to control mice, which makes this a promising base for further investigation.
Izvorni jezik
Engleski
Znanstvena područja
Kemija, Biologija, Farmacija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)
POVEZANOST RADA
Projekti:
IP-2018-01-7683 - Analiza interakcija butirilkolinesteraze s novim inhibitorima i reaktivatorima (AnalyseBChE) (Kovarik, Zrinka, HRZZ - 2018-01) ( CroRIS)
--IP-2016-06-8636 - Molekularni biljezi vulnerabilnosti, adaptacije i plastičnosti neurona u akutnoj i kroničnoj ozljedi mozga (NeuroReact) (Kalanj-Bognar, Svjetlana) ( CroRIS)
Ustanove:
Institut za medicinska istraživanja i medicinu rada, Zagreb,
Medicinski fakultet, Zagreb
Profili:
Kristina Mlinac Jerković (autor)
Borna Puljko (autor)
Svjetlana Kalanj-Bognar (autor)
Katarina Ilić (autor)
Zrinka Kovarik (autor)
Nikolina Macek Hrvat (autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE