Pregled bibliografske jedinice broj: 1208463
Liraglutide Exerts Protective Effects by Downregulation of PPARγ, ACSL1 and SREBP-1c in Huh7 Cell Culture Models of Non-Alcoholic Steatosis and Drug-Induced Steatosis
Liraglutide Exerts Protective Effects by Downregulation of PPARγ, ACSL1 and SREBP-1c in Huh7 Cell Culture Models of Non-Alcoholic Steatosis and Drug-Induced Steatosis // Current issues in molecular biology, 44 (2022), 8; 3465-3480 doi:10.3390/cimb44080239 (međunarodna recenzija, članak, znanstveni)
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Naslov
Liraglutide Exerts Protective Effects by
Downregulation of PPARγ, ACSL1 and SREBP-1c in
Huh7 Cell Culture Models of Non-Alcoholic
Steatosis and Drug-Induced Steatosis
Autori
Omanovic Kolaric, Tea ; Kizivat, Tomislav ; Nincevic, Vjera ; Zjalic, Milorad ; Bilic- Curcic, Ines ; Kuna, Lucija ; Smolic, Robert ; Vcev, Aleksandar ; Wu, George ; Smolic, Martina
Izvornik
Current issues in molecular biology (1467-3037) 44
(2022), 8;
3465-3480
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
NAS ; amiodarone ; liraglutide ; Huh7 cell line
Sažetak
(1) Background: With the aging of the population and polypharmacy encountered in the elderly, drug- induced steatosis (DIS) has become frequent cause of non-alcoholic steatosis (NAS). Indeed, NAS and DIS may co-exist, making the ability to distinguish between the entities ever more important. The aim of our study was to study cell culture models of NAS and DIS and determine the effects of liraglutide (LIRA) in those models. (2) Methods: Huh7 cells were treated with oleic acid (OA), or amiodarone (AMD) to establish models of NAS and DIS, respectively. Cells were treated with LIRA and cell viability was assessed by MTT, lipid accumulation by Oil-Red-O staining and triglyceride assay, and intracellular signals involved in hepatosteatosis were quantitated by RT-PCR. (3) Results: After exposure to various OA and AMD concentrations, those that achieved 80% of cells viabilities were used in further experiments to establish NAS and DIS models using 0.5 mM OA and 20 MAMD, respectively. In both models, LIRA increased cell viability (p < 0.01). Lipid accumulation was increased in both models, with microsteatotic pattern in DIS, and macrosteatotic pattern in NAS which corresponds to greater triglyceride accumulation in latter. LIRA ameliorated these changes (p < 0.001), and downregulated expression of lipogenic ACSL1, PPARg, and SREBP-1c pathways in the liver (p < 0.01) (4) Conclusions: LIRA ameliorates hepatocyte steatosis in Huh7 cell culture models of NAS and DIS.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti
POVEZANOST RADA
Projekti:
NadSve-Sveučilište J. J. Strossmayera u Osijeku-VIF 2019 - Procjena staničnih modela za poboljšanu predikciju lijekovima uzrokovane masne promjene jetre (Smolić, Martina, NadSve ) ( CroRIS)
NadSve-Sveučilište J. J. Strossmayera u Osijeku-VIF 2021 - Osobitosti izražaja biljega i gena endotelne disfunkcije u bolesnika sa šećernom bolesti tipa 2 liječenih SGLT2 inhibitorima (Smolić, Martina, NadSve ) ( CroRIS)
MZO Ustanova-Fakultet za dentalnu medicinu i zdravstvo, Osijek -IP8 - Povezanost biljega lipogeneze i mitohondrijske disfunkcije s individualnim razlikama pri nastanku lijekovima izazvanog jetrenog oštećenja (Smolić, Robert, MZO Ustanova ) ( CroRIS)
Ustanove:
Medicinski fakultet, Rijeka,
Klinički bolnički centar Osijek,
Medicinski fakultet, Osijek,
Fakultet za dentalnu medicinu i zdravstvo, Osijek
Profili:
Robert Smolić
(autor)
Vjera Mihaljević
(autor)
Ines Bilić-Ćurčić
(autor)
Milorad Zjalić
(autor)
Lucija Kuna Roguljić
(autor)
Tomislav Kizivat
(autor)
Tea Omanović Kolarić
(autor)
Aleksandar Včev
(autor)
Martina Smolić
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE