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Pregled bibliografske jedinice broj: 1208463

Liraglutide Exerts Protective Effects by Downregulation of PPARγ, ACSL1 and SREBP-1c in Huh7 Cell Culture Models of Non-Alcoholic Steatosis and Drug-Induced Steatosis


Omanovic Kolaric, Tea; Kizivat, Tomislav; Nincevic, Vjera; Zjalic, Milorad; Bilic- Curcic, Ines; Kuna, Lucija; Smolic, Robert; Vcev, Aleksandar; Wu, George; Smolic, Martina
Liraglutide Exerts Protective Effects by Downregulation of PPARγ, ACSL1 and SREBP-1c in Huh7 Cell Culture Models of Non-Alcoholic Steatosis and Drug-Induced Steatosis // Current issues in molecular biology, 44 (2022), 8; 3465-3480 doi:10.3390/cimb44080239 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 1208463 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Liraglutide Exerts Protective Effects by Downregulation of PPARγ, ACSL1 and SREBP-1c in Huh7 Cell Culture Models of Non-Alcoholic Steatosis and Drug-Induced Steatosis

Autori
Omanovic Kolaric, Tea ; Kizivat, Tomislav ; Nincevic, Vjera ; Zjalic, Milorad ; Bilic- Curcic, Ines ; Kuna, Lucija ; Smolic, Robert ; Vcev, Aleksandar ; Wu, George ; Smolic, Martina

Izvornik
Current issues in molecular biology (1467-3037) 44 (2022), 8; 3465-3480

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
NAS ; amiodarone ; liraglutide ; Huh7 cell line

Sažetak
(1) Background: With the aging of the population and polypharmacy encountered in the elderly, drug- induced steatosis (DIS) has become frequent cause of non-alcoholic steatosis (NAS). Indeed, NAS and DIS may co-exist, making the ability to distinguish between the entities ever more important. The aim of our study was to study cell culture models of NAS and DIS and determine the effects of liraglutide (LIRA) in those models. (2) Methods: Huh7 cells were treated with oleic acid (OA), or amiodarone (AMD) to establish models of NAS and DIS, respectively. Cells were treated with LIRA and cell viability was assessed by MTT, lipid accumulation by Oil-Red-O staining and triglyceride assay, and intracellular signals involved in hepatosteatosis were quantitated by RT-PCR. (3) Results: After exposure to various OA and AMD concentrations, those that achieved 80% of cells viabilities were used in further experiments to establish NAS and DIS models using 0.5 mM OA and 20 MAMD, respectively. In both models, LIRA increased cell viability (p < 0.01). Lipid accumulation was increased in both models, with microsteatotic pattern in DIS, and macrosteatotic pattern in NAS which corresponds to greater triglyceride accumulation in latter. LIRA ameliorated these changes (p < 0.001), and downregulated expression of lipogenic ACSL1, PPARg, and SREBP-1c pathways in the liver (p < 0.01) (4) Conclusions: LIRA ameliorates hepatocyte steatosis in Huh7 cell culture models of NAS and DIS.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti



POVEZANOST RADA


Projekti:
NadSve-Sveučilište J. J. Strossmayera u Osijeku-VIF 2019 - Procjena staničnih modela za poboljšanu predikciju lijekovima uzrokovane masne promjene jetre (Smolić, Martina, NadSve ) ( CroRIS)
NadSve-Sveučilište J. J. Strossmayera u Osijeku-VIF 2021 - Osobitosti izražaja biljega i gena endotelne disfunkcije u bolesnika sa šećernom bolesti tipa 2 liječenih SGLT2 inhibitorima (Smolić, Martina, NadSve ) ( CroRIS)
MZO Ustanova-Fakultet za dentalnu medicinu i zdravstvo, Osijek -IP8 - Povezanost biljega lipogeneze i mitohondrijske disfunkcije s individualnim razlikama pri nastanku lijekovima izazvanog jetrenog oštećenja (Smolić, Robert, MZO Ustanova ) ( CroRIS)

Ustanove:
Medicinski fakultet, Rijeka,
Klinički bolnički centar Osijek,
Medicinski fakultet, Osijek,
Fakultet za dentalnu medicinu i zdravstvo, Osijek

Poveznice na cjeloviti tekst rada:

Pristup cjelovitom tekstu rada doi www.mdpi.com

Citiraj ovu publikaciju:

Omanovic Kolaric, Tea; Kizivat, Tomislav; Nincevic, Vjera; Zjalic, Milorad; Bilic- Curcic, Ines; Kuna, Lucija; Smolic, Robert; Vcev, Aleksandar; Wu, George; Smolic, Martina
Liraglutide Exerts Protective Effects by Downregulation of PPARγ, ACSL1 and SREBP-1c in Huh7 Cell Culture Models of Non-Alcoholic Steatosis and Drug-Induced Steatosis // Current issues in molecular biology, 44 (2022), 8; 3465-3480 doi:10.3390/cimb44080239 (međunarodna recenzija, članak, znanstveni)
Omanovic Kolaric, T., Kizivat, T., Nincevic, V., Zjalic, M., Bilic- Curcic, I., Kuna, L., Smolic, R., Vcev, A., Wu, G. & Smolic, M. (2022) Liraglutide Exerts Protective Effects by Downregulation of PPARγ, ACSL1 and SREBP-1c in Huh7 Cell Culture Models of Non-Alcoholic Steatosis and Drug-Induced Steatosis. Current issues in molecular biology, 44 (8), 3465-3480 doi:10.3390/cimb44080239.
@article{article, author = {Omanovic Kolaric, Tea and Kizivat, Tomislav and Nincevic, Vjera and Zjalic, Milorad and Bilic- Curcic, Ines and Kuna, Lucija and Smolic, Robert and Vcev, Aleksandar and Wu, George and Smolic, Martina}, year = {2022}, pages = {3465-3480}, DOI = {10.3390/cimb44080239}, keywords = {NAS, amiodarone, liraglutide, Huh7 cell line}, journal = {Current issues in molecular biology}, doi = {10.3390/cimb44080239}, volume = {44}, number = {8}, issn = {1467-3037}, title = {Liraglutide Exerts Protective Effects by Downregulation of PPARγ, ACSL1 and SREBP-1c in Huh7 Cell Culture Models of Non-Alcoholic Steatosis and Drug-Induced Steatosis}, keyword = {NAS, amiodarone, liraglutide, Huh7 cell line} }
@article{article, author = {Omanovic Kolaric, Tea and Kizivat, Tomislav and Nincevic, Vjera and Zjalic, Milorad and Bilic- Curcic, Ines and Kuna, Lucija and Smolic, Robert and Vcev, Aleksandar and Wu, George and Smolic, Martina}, year = {2022}, pages = {3465-3480}, DOI = {10.3390/cimb44080239}, keywords = {NAS, amiodarone, liraglutide, Huh7 cell line}, journal = {Current issues in molecular biology}, doi = {10.3390/cimb44080239}, volume = {44}, number = {8}, issn = {1467-3037}, title = {Liraglutide Exerts Protective Effects by Downregulation of PPARγ, ACSL1 and SREBP-1c in Huh7 Cell Culture Models of Non-Alcoholic Steatosis and Drug-Induced Steatosis}, keyword = {NAS, amiodarone, liraglutide, Huh7 cell line} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati:





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