Naslov
Regulation of ssb gene expression in Escherichia coli

Autori
Feliciello, Isidoro ; Đermić, Edyta ; Davor Zahradka, Davor ; Ivanković, Siniša ; Đermić Damir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
FEMS Conference on Microbiology : Electronic abstract book / - Belgrade, 2022, 125-125

ISBN
978-86-914897-8-6

Skup
2nd FEMS Conference on Microbiology

Mjesto i datum
Beograd, Srbija, 30.06.2022. - 02.07.2022

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
SOS induction

Sažetak
Bacterial SSB proteins, as well as their eukaryotic RPA analogues, are essential and ubiquitous. They bind single-strand DNA and regulate/coordinate its metabolism, hence enabling essential processes such as DNA replication, DNA transcription and DNA repair. The prototypic SSB protein, the one of Escherichia coli, is encoded by an ssb gene. Although the ssb gene promoter harbors SOS box, multiple studies during several decades failed to elucidate the mechanisms of the ssb gene regulation, i.e. whether it is inducible and SOS dependent. SOS regulon is comprised of about 40 genes, whose transcription is induced in stress conditions in a coordinated way. Using quantitative real-time PCR, we determined the ssb gene expression in UV- and γ-irradiated E. coli and revealed that the ssb gene expression is elevated in irradiated cells in an SOS-dependent manner. Elucidating regulation of expression of the essential bacterial gene such as the ssb can be very useful for understanding regulation of the above mentioned essential DNA metabolic processes, as well as for finding ways of combating bacterial infections.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Biotehnologija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA