Naslov
Shewanella spp. from wastewater treatment plant‑afected environment: isolation and characterization

Autori
Topić Popović, Natalija ; Kazazić, Snježana ; Bilić, Branka ; Babić, Sanja ; Bojanić, Krunoslav ; Bujak, Maro ; Tartaro Bujak, Ivana ; Jadan, Margita ; Strunjak-Perović, Ivančica ; Kepec, Slavko ; Čož- Rakovac, Rozelinda

Izvornik
Environmental science and pollution research (0944-1344) 29 (2022), 55; 82986-83003

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Antimicrobial resistance ; Biofilm ; Fatty acid profile ; Identification ; MALDI-TOF MS ; Shewanella ; WWTP ; Zebrafish

Sažetak
Bacteria from the genus Shewanella are inhabitants of marine and freshwater ecosystems, recognized fish spoilage bacteria, but less known as fish disease agents. Shewanella spp. isolated from fish living in waters close to effluents of a wastewater treatment plant (WWTP) were not previously characterized. We have tested Shewanella isolates from WWTP-affected waters and related fish. Genotypic characterization identified most strains as S. baltica and S. oneidensis. In order to investigate the sensibility and accuracy of their MALDI-TOF MS identification, they were grown on two culture media enriched by various NaCl concentrations, incubated at different temperatures and duration. We analyzed their antimicrobial susceptibility on a panel of antimicrobial drugs and capacity for biofilm production. With a view to demonstrate their capacity to produce fatty acids, we assessed the impact of different culture media on their lipid profile. We performed zebrafish embryotoxicity tests to simulate the environmental infection of the earliest life stages in S. baltica-contaminated waters. The best MALDI-TOF MS identification scores were for strains cultivated on TSA for 24 h at 22 °C and with supplementation of 1.5% NaCl. Less than 17% of isolates demonstrated antimicrobial resistance. Most isolates were weak biofilm producers. Strain-to- strain variation of MIC and MBC was low. The major fatty acids were C15:0, C16:0, C16:1, C17:1, and iC15:0. Exposure of Danio rerio to different S. baltica concentrations induced severe effects on zebrafish development: decreased heartbeat rate, locomotor activity, and melanin pigmentation. S. baltica passed through chorionic pores of zebrafish.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Veterinarska medicina, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA