Naslov
Poly (I:C) stimulation influences the expression of calreticulin, profilin-1 and heterogenous nuclear ribonucleoprotein A1 in a human HNSCC cell line: a proteomic study

Autori
Matijević Glavan, Tanja ; Pavelić, Jasminka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
European journal of cancer: From basic research to personalized cancer treatment / Eggermont, Alexander M. M. - Oxford : Elsevier, 2012, S29-S29

Skup
22nd Bienal Congress of the European Association for Cancer Research

Mjesto i datum
Barcelona, Španjolska, 07.07.2012. - 10.07.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
poly (I:C), HNSCC

Sažetak
Introduction. Poly(I:C) has been formerly known to be an interferon inducer but the mechanism of its action was not revealed until the discovery of Toll-like receptors (TLRs). TLRs are transmembrane proteins that recognize conserved molecular motifs of viral and bacterial origin and initiate innate immune response. Recent studies have shown that they are also expressed on tumor cells but their role in these cells is still not clear. TLR3 recognizes double stranded RNA (poly(I:C)) and is primarily involved in the defense against viruses similarly as other two cytoplasmic receptors, melanoma differentiation associated gene 5 (MDA5) and retinoic acid inducible gene I (RIG-I), that also recognize dsRNA. Poly (I:C) binding initiates the activation of transcription factors NF-ĸB, IRF family members and AP-1, which can induce wide cascading effect on the cell and consequently activate many cellular processes. Material and methods. In order to widen the current knowledge of TLR3 target genes, we have treated HNSCC cell line Detroit 562 with poly (I:C) and used the proteomic approach. We have confirmed the obtained results by western blot. Results and discussion. In this study we have discovered 15 differentially expressed proteins, mostly connected with protein metabolic processes. Furthermore, we have confirmed that calreticulin, profilin-1 and heterogeneous nuclear ribonucleoprotein A1 (HNRNPA1), proteins which have been shown previously to be involved in processes connected with tumor progression, are differentially expressed after poly (I:C) stimulation. In addition, we have shown that HNRNPA1 is noticeably over-expressed after the inhibition of MDA5 and RIG-I expression. Conclusion. Since poly (I:C) has been studied and utilized in tumor therapy as an immunostimulatory adjuvant, our results indicate that further studies are needed before the clinical application in order to prevent possible side effects.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA