Naslov
Control of Rrp6 stability in yeast sporulation

Autori
Novačić, Ana ; Štrbac, Lucija ; Becker, Emmanuelle ; Law, Michael ; Primig, Michael ; Stuparević, Igor

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Programme and Abstract Book of the IUBMB-FEBS-PABMB Young Scientists' Forum and the FEBS 2022 Biochemistry Global Summit / Salome Veiga, Ana ; Domingues, Marco ; Rocha, Barbara ; Lousa, Diana ; Fernandes, Fabio - Lisabon, 2022, 152-152

Skup
The Biochemistry Global Summit

Mjesto i datum
Lisabon, Portugal, 06.07.2022. - 14.07.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
RNA exosome, Rrp6, meiosis, proteolysis, APC

Sažetak
The conserved 3'-5' exoribonuclease Rrp6 (EXOSC10 in mammals) is a catalytic subunit of the nuclear RNA exosome, which degrades and processes RNAs in eukaryotic cells. Rrp6 and EXOSC10 are important for efficient meiotic development in yeast and male and female gametogenesis in mammals, respectively. We have shown earlier that yeast Rrp6 protein levels decrease during meiotic M-phase and yeast spore formation although the mRNA continues to be expressed. Given that Rrp6/EXOSC10 undergoes a variety of post-translational modifications, including ubiquitination, we hypothesized that Rrp6 is degraded by targeted proteolysis in differentiating cells. We first confirm that Rrp6 becomes unstable when cells progress from fermentation to respiration and sporulation and then show that the protein rapidly accumulates during initial rounds of cell division after spore germination. Furthermore, we show that Rrp6 degradation requires an active Anaphase Promoting Complex/Cyclosome (APC/C). Finally, we demonstrate that an Rrp6 allele lacking the sole APC/C target motif (destruction box) conserved from yeast to humans remains detectable during sporulation. We conclude that Rrp6 protein stability is under nutritional control via targeted proteolysis by the APC/C-proteasome pathway. These results are relevant for mammals because EXOSC10 is also ubiquitinated, diminishes during spermatogenesis and becomes unstable after cold-shock via SUMOylation.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Biotehnologija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

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