Naslov
Activation of NLRP3 inflammasome in stable chronic obstructive pulmonary disease

Autori
Markelić, Ivona ; Hlapčić, Iva ; Ceri, Andrea ; Radic Antolic, Margareta ; Samaržija, Miroslav ; Popović-Grle, Sanja ; Vukić Dugac, Andrea ; Rumora, Lada

Izvornik
Scientific reports (2045-2322) 12 (2022); 7544, 8

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
chronic obstructive pulmonary disease ; Interleukin-1beta ; NLRP3 inflammasome ; Gene expression

Sažetak
Nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome activation plays an important role in chronic obstructive pulmonary disease (COPD) pathogenesis and might be involved in ongoing chronic inflammation. This study aimed to determine interleukin-1beta (IL-1β) plasma concentration as well as IL1B, NLRP3 and caspase-1 (CASP1) gene expression in the Croatian COPD patients. 109 patients with stable COPD and age- and sex-matched 95 controls were included in the study. Plasma IL-1β concentration was measured by Luminex technology, and gene expression analysis was performed using TaqMan assays. It was shown that COPD patients had increased concentration of IL-1β and enhanced gene expression of IL1B, NLRP3 and CASP1 compared to controls. There was no difference in IL-1β or IL1B, NLRP3 and CASP1 in patients with COPD regarding airflow obstruction severity and smoking history. Finally, the diagnostic potential of the determined parameters was evaluated, and it was found that IL-1β correctly classified 89% of cases in the combination with common inflammatory biomarkers, white blood cell count and fibrinogen, showing a potential in COPD prediction. In conclusion, up- regulation of IL1B, NLRP3, CASP1 and increased IL- 1β concentration suggest the activation of NLRP3 inflammasome in the systemic compartment of patients with stable COPD.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti, Farmacija

POVEZANOST RADA