Naslov
Neuronal laminar identity markers delineate regional differences during the primate-specific subplate expansion period in the human fetal cortex

Autori
Junaković, Alisa ; Kopić, Janja ; Duque, Alvaro ; Kostović, Ivica ; Krsnik, Željka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstract E-book FENS 2022 / - , 2022, 2533-2533

Skup
13th FENS Forum of Neuroscience

Mjesto i datum
Pariz, Francuska, 09.07.2022. - 13.07.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
human brain development ; cerebral cortex ; subplate

Sažetak
Development of the cortical anlage begins with the formation of the preplate (mantle by His), which is split by the first cohort of cortical plate (CP) cells in that way the specific marginal zone (MZ) containing Cajal-Retzius neurons (CRN) and presubplate (P-SP) are formed. The period between 13 and 15 postconceptional weeks (PCW) is characterized by a transformation (cell spread- down) of the deep portion of the CP. During this human-characteristic event, thalamocortical and basal forebrain afferent fibers penetrate tangentially and obliquely in the CP. In that way, cells of the deep portion of the CP are secondarily displaced downwards („second“ CP) forming the future expanded subplate (SP). Future SP neurons are born in the proliferative zones simultaneously as prospective cortical layer VI neurons. Importantly, future layer VI neurons are not secondarily dispersed during the SP formation period. Thus, our study aimed to show regional expression patterns of specific transcription factors, i.e. projection neurons markers, as well as synaptic markers with the special emphasis on the SP expansion period utilizing immunofluorescence. Our results show that markers of projection neurons demonstrate characteristic regional differences in the neocortical part of the cerebral wall during the process of SP expansion. We concluded that projection neurons markers of cortical layers V, VI, and projection neuronal population of prospective glutamatergic SP neurons are the key players in the establishment of early regional differences of the neocortex during the SP formation period.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

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