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Pregled bibliografske jedinice broj: 1205443

The Role of ATF3 in Neuronal Differentiation and Development of Neuronal Networks in Opossum Postnatal Cortical Cultures


Petrović, Antonela; Ban, Jelena; Ivaničić, Matea; Tomljanović, Ivana; Mladinic, Miranda
The Role of ATF3 in Neuronal Differentiation and Development of Neuronal Networks in Opossum Postnatal Cortical Cultures // International Journal of Molecular Sciences, 23 (2022), 9; 4964, 22 doi:10.3390/ijms23094964 (međunarodna recenzija, članak, znanstveni)


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Naslov
The Role of ATF3 in Neuronal Differentiation and Development of Neuronal Networks in Opossum Postnatal Cortical Cultures

Autori
Petrović, Antonela ; Ban, Jelena ; Ivaničić, Matea ; Tomljanović, Ivana ; Mladinic, Miranda

Izvornik
International Journal of Molecular Sciences (1422-0067) 23 (2022), 9; 4964, 22

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
opossums ; neuronal differentiation ; neuroregeneration ; neurospheres

Sažetak
Activating transcription factor 3 (ATF3), a member of the ATF/cAMP response element-binding (CREB) family, is upregulated by various intracellular and extracellular signals such as injury and signals related to cell proliferation. ATF3 also belongs to the regeneration-associated genes (RAG) group of transcription factors. RAG and ATF/CREB transcription factors that play an important role in embryonic neuronal development and PNS regeneration may also be involved in postnatal neuronal differentiation and development, as well as in the regeneration of the injured CNS. Here we investigated the effect of ATF3 in differentiation, neural outgrowth, network formation, and regeneration after injury using postnatal dissociated cortical neurons derived from neonatal opossums (Monodelphis domestica). Our results show that RAG and ATF genes are differentially expressed in early differentiated neurons versus undifferentiated neurospheres and that many members of those families, ATF3 in particular, are upregulated in cortical cultures obtained from younger animals that have the ability to fully functionally regenerate spinal cord after injury. In addition, we observed different intracellular localization of ATF3 that shifts from nuclear (in neuronal progenitors) to cytoplasmic (in more mature neurons) during neuronal differentiation. The ATF3 inhibition, pharmacological or by specific antibody, reduced the neurite outgrowth and differentiation and caused increased cell death in early differentiating cortical neuronal cultures, suggesting the importance of ATF3 in the CNS development of neonatal opossums. Finally, we investigated the regeneration capacity of primary cortical cultures after mechanical injury using the scratch assay. Remarkably, neonatal opossum- derived cultures retain their capacity to regenerate for up to 1 month in vitro. Inhibition of ATF3 correlates with reduced neurite outgrowth and regeneration after injury. These results indicate that ATF3, and possibly other members of RAG and ATF/CREB family of transcription factors, have an important role both during cortical postnatal development and in response after injury.

Izvorni jezik
Engleski



POVEZANOST RADA


Projekti:
HRZZ-IP-2016-06-7060 - Istraživanje granice između neurodegeneracije i neuroregeneracije: identifikacija ključnih molekula pomoću proteomike i funkcionalnih testova na leđnoj moždini sisavaca (DefineREgenAgeMode) (Mladinić Pejatović, Miranda, HRZZ - 2016-06) ( CroRIS)
NadSve-Sveučilište u Rijeci-uniri-prirod-18-290 1463 - Identifikacija i kvantifikacija neuralnih matičnih stanica novom metodom optimizirane homogenizacije (Ban, Jelena, NadSve - UNIRI projekti 2018) ( CroRIS)
NadSve-Sveučilište u Rijeci-uniri-biomed-18-258 6427 - Otkrivanje ključnih molekula koje kontroliraju stanične mehanizme neuroprotekcije i neuroregeneracije posredovane proteinima toplinskog stresa nakon in vitro ozljede leđne moždine oposuma (Mladinić Pejatović, Miranda, NadSve - UNIRI projekti 2018) ( CroRIS)

Ustanove:
Sveučilište u Rijeci - Odjel za biotehnologiju

Citiraj ovu publikaciju:

Petrović, Antonela; Ban, Jelena; Ivaničić, Matea; Tomljanović, Ivana; Mladinic, Miranda
The Role of ATF3 in Neuronal Differentiation and Development of Neuronal Networks in Opossum Postnatal Cortical Cultures // International Journal of Molecular Sciences, 23 (2022), 9; 4964, 22 doi:10.3390/ijms23094964 (međunarodna recenzija, članak, znanstveni)
Petrović, A., Ban, J., Ivaničić, M., Tomljanović, I. & Mladinic, M. (2022) The Role of ATF3 in Neuronal Differentiation and Development of Neuronal Networks in Opossum Postnatal Cortical Cultures. International Journal of Molecular Sciences, 23 (9), 4964, 22 doi:10.3390/ijms23094964.
@article{article, author = {Petrovi\'{c}, Antonela and Ban, Jelena and Ivani\v{c}i\'{c}, Matea and Tomljanovi\'{c}, Ivana and Mladinic, Miranda}, year = {2022}, pages = {22}, DOI = {10.3390/ijms23094964}, chapter = {4964}, keywords = {opossums, neuronal differentiation, neuroregeneration, neurospheres}, journal = {International Journal of Molecular Sciences}, doi = {10.3390/ijms23094964}, volume = {23}, number = {9}, issn = {1422-0067}, title = {The Role of ATF3 in Neuronal Differentiation and Development of Neuronal Networks in Opossum Postnatal Cortical Cultures}, keyword = {opossums, neuronal differentiation, neuroregeneration, neurospheres}, chapternumber = {4964} }
@article{article, author = {Petrovi\'{c}, Antonela and Ban, Jelena and Ivani\v{c}i\'{c}, Matea and Tomljanovi\'{c}, Ivana and Mladinic, Miranda}, year = {2022}, pages = {22}, DOI = {10.3390/ijms23094964}, chapter = {4964}, keywords = {opossums, neuronal differentiation, neuroregeneration, neurospheres}, journal = {International Journal of Molecular Sciences}, doi = {10.3390/ijms23094964}, volume = {23}, number = {9}, issn = {1422-0067}, title = {The Role of ATF3 in Neuronal Differentiation and Development of Neuronal Networks in Opossum Postnatal Cortical Cultures}, keyword = {opossums, neuronal differentiation, neuroregeneration, neurospheres}, chapternumber = {4964} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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