Naslov
Gender differences in expression of rat renal OAT1

Autori
Burckhardt, Gerhard ; Ljubojević, Marija ; Herak-Kramberger, Carol M. ; Hagos, Yohannes ; Bahn, Andrew ; Endou, Hitoshi ; Sabolić, Ivan

Izvornik
Pflügers Archiv (0031-6768) 445 (2003), Suppl.1;

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, kongresno priopcenje, znanstveni

Ključne riječi
organic anion transporters; sex differences; testosterone; estrogen

Sažetak
The gender differences (GD) in the renal secretion of organic anions may be due to different expression levels of the organic anion transporter 1 (OAT1) in the basolateral membrane (BLM) of the S2 proximal tubule segment. By using immunofluorescence cytochemistry in cryosections of fixed renal tissue and immunoblotting of isolated renal cortical BLM and total cell membranes, we studied localization and the abundance of OAT1 in adult and prepubertal male (M) and female (F) rats, as well as an effect of gonadectomy and steroid replacement therapy. The expression of OAT1 protein in the S2 segment was stronger in M than in F. In prepubertal rats, OAT1 abundance was low and exhibited no GD. In adult M, the abundance of OAT1 decreased by 45% two weeks after castration, whereas daily s.c. treatment of castrated M with testosterone, estradiol, or progesterone (2.5 mg/kg b.m. each) for 8 days resulted in a complete restitution, further (88%) depression, or overexpression (22%) of OAT1, respectively. In F rats, ovariectomy had no effect, but additional treatment with estradiol caused a strong decrease (73%) of OAT1. Immunoblotting data completely matched the findings by immunofluorescence. We conclude that GD in renal OAT1 expression are due to stimulation by androgens and progesterone, and inhibition by estrogens.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA