Naslov
The Role of S-Layer Proteins in Probiotic Properties of Levilactobacillus brevis Strains Isolated from Human Milk Microbiota

Autori
Butorac, Katarina ; Novak, Jasna ; Leboš Pavunc, Andreja ; Banić, Martina ; Čuljak, Nina ; Kazazić, Saša ; Kazazić, Snježana ; Šušković, Jagoda ; Kos, Blaženka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts from "7th Croatian Congress of Microbiology with International Participation" / Sviličić Petrić, Ines ; Leboš Pavunc, Andreja ; Šantić, Marina ; Kifer, Domagoj - Zagreb : Recedo digital, 2022, 111-111

ISBN
978-953-7778-18-7

Skup
7th Croatian Congress of Microbiology

Mjesto i datum
Sveti Martin na Muri, Hrvatska, 24.05.2022. - 27.05.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
breast milk microbiota ; Levilactobacillus brevis ; S-proteins

Sažetak
Infant’s intestinal microbiome is believed to be highly influenced by their own mother’s breast milk microbiota. Mother’s milk is a rich source of nutrients, bioactive components, but also a diversity of microorganisms that are important for the health of a newborn. The focus of our research was screening of the Lactobacillus strains that rarely, but preferably, express S-proteins. Among over 100 strains, we have identified S-proteins solely in the 4 strains of Levilactobacillus spp. This is the first report that describes S-proteins of Levilactobacillus brevis strains isolated from mother’s milk. Four L. brevis strains, MB1, MB2, MB13 and MB20, were identified by whole genome sequencing. Gene annotation confirmed the presence of S-layer proteins, which were further identified by SDS-PAGE coupled to LC-MS/MS. The examined L. brevis strains possess S-proteins of 40-50 kDa. Further characterization revealed that S-proteins protect bacterial cells against adverse environmental stresses, such as those in gastrointestinal tract (GIT) or during freeze- drying, but also interact during cell adhesion to intestinal surfaces. This was observed through increased survival of the L. brevis strains in simulated GIT conditions, increased adhesion to Caco-2 cell line and stronger inhibition of adhesion of pathogenic bacterium Escherichia coli 3014 to Caco-2 cell line, as mentioned properties were negatively affected after the removal of S- layer proteins from the cell surface. Results imply that S-proteins mediate functional properties of the producer strains. Overall, S- layer proteins of investigated L. brevis strains have the potential to act as key mediators for their functionalities.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

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