Pregled bibliografske jedinice broj: 1202671
Long read sequencing characterization of intra-host viral variants following vertical transmission of Cryphonectria hypovirus 1
Long read sequencing characterization of intra-host viral variants following vertical transmission of Cryphonectria hypovirus 1 // 7th Croatian Congress of Microbiology with International Participation - Book of Abstracts
Sveti Martin na Muri, Hrvatska, 2022. str. 40-40 (predavanje, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 1202671 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Long read sequencing characterization of intra-host viral variants following vertical transmission of Cryphonectria hypovirus 1
Autori
Peranić, Karla ; Leigh, Deborah M ; Morić, Maja ; Nuskern, Lucija ; Ćurković-Perica, Mirna ; Kupper, Quirin ; Cornejo, Carolina ; Rigling, Daniel ; Ježić, Marin
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
7th Croatian Congress of Microbiology with International Participation - Book of Abstracts
/ - , 2022, 40-40
Skup
7th Croatian Congress of Microbiology
Mjesto i datum
Sveti Martin na Muri, Hrvatska, 24.05.2022. - 27.05.2022
Vrsta sudjelovanja
Predavanje
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
none
Sažetak
Cryphonectria hypovirus 1 (CHV1) acts as an attenuating agent of Cryphonectria parasitica, an ascomycete fungus which causes chestnut blight, a devastating disease of the chestnuts. Transmission of virus is possible via asexual spores into the progeny (vertical transmission) and through hyphal anastomosis between individuals (horizontal transmission). Previous research showed that vertical transmission of the virus depends significantly on CHV1 subtype and strain. On average, transmission of the virus was observed into 69% of the asexual spores (conidia). In our experiment we used two different subtypes of CHV1: subtype I, the most widespread across Europe and naturally present in Croatia and subtype F, which has a stronger effect on its host (more strongly inhibits host’s ability to grow and sporulate than subtype I). The percentage of CHV1 transmission into the conidia was high (over 75%) and didn’t differ between the two subtypes. Since genomic sequences are the most reliable and comprehensive source of information about viruses and their evolution, we analysed intra-host CHV1 diversity utilising PacBio (Pacific Biosciences) sequencing which generates long reads with high consensus accuracy and a uniform coverage. We studied the mutational rate and change of haplotypic diversity between the parent isolate and its spores. We observed a significant decrease in mutational diversity, however, there was no statistically significant decrease in haplotypic diversity. The bottleneck effect during the vertical virus transmission is visible, but it appears that the overall haplotypic diversity is inherited by the spores.
Izvorni jezik
Engleski
Znanstvena područja
Biologija
POVEZANOST RADA
Projekti:
HRZZ-IZHRZ0_180651 - Dynamics of virus infection in mycovirus-mediated biological control of a fungal pathogen (DynaMyco) (Ježić, Marin, HRZZ - Croatian-Swiss Research Programme 2017 - 2023) ( CroRIS)
Ustanove:
Prirodoslovno-matematički fakultet, Zagreb
Profili:
Mirna Ćurković Perica
(autor)
Marin Ježić
(autor)
Maja Popović
(autor)
Karla Peranić
(autor)
Lucija Nuskern Karaica
(autor)