Pregled bibliografske jedinice broj: 1202021
CHANGES OF VASCULAR REACTIVITY TO ADENOSINE A1 AND A2A RECEPTOR AGONISTS AFTER HYPERBARIC OXYGENATION
CHANGES OF VASCULAR REACTIVITY TO ADENOSINE A1 AND A2A RECEPTOR AGONISTS AFTER HYPERBARIC OXYGENATION // Journal of Hypertension: June 2022 - Volume 40 - Issue Suppl 1 - p e266 / Heagerty, Anthony M. (ur.).
Atena, Grčka: Wolters Kluwer, 2022. str. e266-e266 doi:10.1097/01.hjh.0000838252.80031.88 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 1202021 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
CHANGES OF VASCULAR REACTIVITY TO ADENOSINE A1 AND
A2A RECEPTOR AGONISTS AFTER HYPERBARIC OXYGENATION
Autori
Đambic, Vedran ; Mihaljević, Zrinka ; Drenjančević, Ines ; Jukić, Ivana ; Kibel, Aleksandar
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Journal of Hypertension: June 2022 - Volume 40 - Issue Suppl 1 - p e266
/ Heagerty, Anthony M. - : Wolters Kluwer, 2022, E266-e266
Skup
31st European Meeting on Hypertension and Cardiovascular Protection
Mjesto i datum
Atena, Grčka, 17.06.2022. - 20.06.2022
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
vascular reactivity, adenosine receptors, hyperbaric oxygenation, Sprague-Dawley rats
Sažetak
Objective: Hyperbaric oxygenation (HBO) affects vascular reactivitiy, which plays an important role in the pathophysiology of hypertension and other cardiovascular diseases. New data suggest important effects of HBO on oxidative stress and alteration of production of vasoactive metabolites in blood vessels ; however, the role of adenosine A1 and A2a receptors is still underinvestigated.This study aimed to determine the functional role of adenosine A1 and A2a receptors in changes of vascular reactivity in isolated middle cerebral arteries (MCA) of Spague- Dawley rats, after acute and intermittent HBO. Desing and Methods: Male and femal healthy Sprague-Dawley rats aged 8-10 weeks were divided into 3 groups: CTRL (control non HBO), A-HBO (single animals exposed to HBO for 2 hours) and 4D-HBO (animals exposed to HBO at a pressure od 2 bars for 2 hours daily for 4 days, with vascularexperiments conducted on the fith day). The rats were anesthetized with ketamine-chloride (3 ml/kg) and midazolam (0, 5 ml/kg) and decapitated. The MCA were isolated, cannulated and pressurized for 60’at 80 mmHg to assess basal diameter using pressure myograph Danish Myo Technology. A1 adenosin receptor selective agonist CCPA or A2a adenosin receptor agonist CGS in concentrations from 10-5 to 10-10 M were added to the vessel chamber, and blood vessel diameter was measured after incubation of the drug for 15 minutes. Statistical analyses were performed with Two-way ANOVA tests ; p<0.05 was considered significant. Results: The vasoconstriction in response to A1 agonist administration was similar between the CTRL and 4D-HBO group, while it was significantly reduced in A-HBO compared to the CTRL and 4D-HBO groups. The vasodilation in response to A2a stimulation was significantly reduced in rats exposed to A-HBO and I-HBO compared to CTRL at higher drug concentrations (10-7 to 10-5 M). Conclusion: Acute exposure of rats to HBO leads to decrease in the vasoconstrictive response to adenosine A1 receptor stimulation, while acute and intermittent exposure of rats to HBO is associated with a decrease in the vasodilatory response of MCA to adenosine A2a receptor activation, suggesting that HBO alters sensitivity to or expression of receptors.
Izvorni jezik
Engleski
Znanstvena područja
Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)
POVEZANOST RADA
Projekti:
NadSve-Sveučilište J. J. Strossmayera u Osijeku-VIF 2021 - Uloga adenozinskih A1 i A2A receptora u mehanizmima vaskularne reaktivnosti nakon hiperbarične oksigenacije - molekularni pristup (Mihaljević, Zrinka, NadSve ) ( CroRIS)
NadSve-Sveučilište J. J. Strossmayera u Osijeku-IP 2020 - Uloga adenozinskih A1 i A2a receptora u promjenama vaskularne reaktivnosti nakon hiperbarične oksigenacije (Kibel, Aleksandar, NadSve ) ( CroRIS)
Ustanove:
Klinički bolnički centar Osijek,
Medicinski fakultet, Osijek
Profili:
Zrinka Mihaljević
(autor)
Ines Drenjančević
(autor)
Aleksandar Kibel
(autor)
Vedran Đambić
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE