Pregled bibliografske jedinice broj: 1202015
PERMISSIVE ROLE OF ANGIOTENSIN II IN MANTENANCE OF ENDOTHELIUM-DEPENDENT VASODILATION IN CEREBRAL MICROCIRCULATION
PERMISSIVE ROLE OF ANGIOTENSIN II IN MANTENANCE OF ENDOTHELIUM-DEPENDENT VASODILATION IN CEREBRAL MICROCIRCULATION // Journal of Hypertension: June 2022 - Volume 40 - Issue Suppl 1 - p e282 / Heagerty, Anthony M. (ur.).
Atena, Grčka: Wolters Kluwer, 2022. str. e282-e282 doi:0.1097/01.hjh.0000838440.67547.4c (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 1202015 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
PERMISSIVE ROLE OF ANGIOTENSIN II IN MANTENANCE OF
ENDOTHELIUM-DEPENDENT VASODILATION IN CEREBRAL
MICROCIRCULATION
Autori
Mihaljević, Zrinka ; Jukić, Ivana ; Matić, Anita ; Stupin, Ana ; Kolobarić, Nikolina ; Kozina, Nataša ; Šušnjara, Petar ; Mihalj, Martina ; Drenjančević, Ines
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Journal of Hypertension: June 2022 - Volume 40 - Issue Suppl 1 - p e282
/ Heagerty, Anthony M. - : Wolters Kluwer, 2022, E282-e282
Skup
31st European Meeting on Hypertension and Cardiovascular Protection
Mjesto i datum
Atena, Grčka, 17.06.2022. - 20.06.2022
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
angiotensin II ; endothelium dependent vasodilation ; cerebral microcirculation ; high salt diet
Sažetak
OBJECTIVE: A number of data suggest, rather controversial, permissive role of angiotensin II (ANGII) in maintaining vasodilator responses in microcirculation, via its interaction with AT1 receptors (AT1R). This study aimed to determine the role of ANGII and AT1R in the mechanisms of flow-induced dilation (FID) in middle cerebral arteries (MCA) and incidence of oxidative stress at the functional, cellular and molecular level in the cerebral vasculature and in the serum of Sprague-Dawley (SD) rats fed high salt diet (HS). DESIGN AND METHOD: Eleven-week old, male SD rats were randomly assigned to control group (CTRL, 0.4% NaCl in rat chow) ; Losartan group (rats on a standard diet given the AT1R blocker losartan (1 mg/mL) in drinking water) ; HS group (7 days 4% NaCl in rat chow) or HS+ANGII group (7 days HS with 3 days ANGII administration via osmotic minipumps (100 ng/kg/min on days 4–7)). FID of MCA was determined in absence/presence of the NOS inhibitor L-NAME, the non-selective cyclooxygenases inhibitor indomethacin, and the superoxide dismutase mimetic TEMPOL. Gene and protein expression of antioxidative enzymes and enzymes involved in FID mechanisms were determined by RT-qPCR and western blot. Vascular NO and superoxide/ROS levels were assessed by direct fluorescence. Serum systemic oxidative stress parameters was measured by spectrophotometry. All experimental procedures conformed to the EU Directive 2010/63/EU. RESULTS: HS or AT1R blockade impaired FID and increased oxidative stress. Supplementation of ANGII restored the mechanisms of FID in MCA of rats fed HS diet to the ones present in CTRL rats without affecting arterial blood pressure by decreasing systemic oxidative stress and decreasing superoxide/ROS levels and increased NO bioavailability in the vascular wall of HS treated rats. The AT1R blockade resulted in significantly attenuated endothelium-dependent dilation, changed mechanisms of FID, increased vascular and systemic oxidative stress and decreased expression/ activity of antioxidative enzymes. CONCLUSION: ANGII is crucial in the preservation of vascular oxidative stress-antioxidant system balance and via AT1R, in maintenance of physiological vasodilation mechanisms of MCA. Suppression of ANGII is a key link between HS intake and development of endothelial dysfunction, and impaired regulation of cerebral blood flow. Funding: This work has been supported by Croatian Science Foundation under the project #IP-2014-09- 6380 (PI Drenjancevic) and in part with VIF-MEFOS- 15 (VIF Drenjančević) and VIF2017-MEFOS-06 (VIF Ćosić) (Faculty of Medicine Osijek, Croatia).
Izvorni jezik
Engleski
Znanstvena područja
Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)
Napomena
Zrinka Mihaljević and Ivana Jukić are equally
contributing authors
POVEZANOST RADA
Projekti:
HRZZ-IP-2014-09-6380 - Poremećena vazorelaksacija i endotelno-leukocitna interakcija (ELI) u razvoju aterosklerotskih lezija (V-ELI Athero) (Drenjančević, Ines, HRZZ - 2014-09) ( CroRIS)
NadSve-Sveučilište J. J. Strossmayera u Osijeku-VIF2017-MEFOS-6 - Utjecaj dijete s visokim udjelom soli na diferencijaciju pomoćnih limfocita T u krvi Sprague Dowley štakora i zdravih mladih ispitanika (Matić, Anita, NadSve ) ( CroRIS)
Ustanove:
Klinički bolnički centar Osijek,
Medicinski fakultet, Osijek
Profili:
Anita Matić
(autor)
Nataša Kozina
(autor)
Zrinka Mihaljević
(autor)
Ines Drenjančević
(autor)
Martina Mihalj
(autor)
Nikolina Bilić-Dujmušić
(autor)
Petar Šušnjara
(autor)
Ana Stupin
(autor)