Naslov
Diversity and antibiotic resistance of bacteria associated to marine plastic litter collected by commercial fishing trawlers in the southern Adriatic Sea, Croatia
(Diversity and antibiotic resistance of bacteria associated to marine plastic litter collected by commercial fishing trawlers in the southern Adriatic Sea, Croatia)

Autori
Kapetanović, Damir ; Vardić Smrzlić, Irena ; Kazazić, Snježana ; Perić, Lorena ; Orlić, Karla ; Filipović Marijić, Vlatka ; Mijošek, Tatjana ; Redžović, Zuzana ; Cukrov, Neven ; Cindrić, Ana-Marija ; Rapljenović, Ana ; Gavrilović, Ana ; Radočaj, Tena

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
7th Croatian Congress of Microbiology with International Participation : Book of abstracts / Sviličić Petrić, Ines ; Laboš Pavunc, Andreja ; Šantić, Marina ; Kifer, Domagoj - Zagreb : Hrvatsko mikrobiološko društvo, 2022, 95-95

ISBN
978-953-7778-18-7

Skup
7th Croatian Congress of Microbiology

Mjesto i datum
Sveti Martin na Muri, Hrvatska, 24.05.2022. - 27.05.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Marine plastic litter ; bacteria, antibiotic resistance, commercial fishing trawlers, Adriatic Sea, Vibrio

Sažetak
Plastic pollution is widespread and well-studied problem, however bacterial colonization of marine plastic litter and its potential detrimental effect on the fish and human health is a relatively new issue. However, recent research suggests that microorganisms living on plastics, known as plastisphere, could be distinctive from the surrounding habitat. There is also a lack of research on marine plastic litter obtained by fishing trawlers in the Adriatic Sea. The aim of the present study was to evaluate the specificities of bacteria associated to plastic litter collected by commercial fishing trawlers in the southern Adriatic Sea (Croatia), with respect to seawater and sediment from the same habitat. Sampling took place in May 2021 along the G5 fishing zone (Neretva Channel) by commercial fishing trawlers. Bacteria from plastic, sediment and seawater samples were isolated on both Marine and TCBS agar plates incubated on 22°C and 35°C and were identified using MALDI TOF Mass Spectrometry, while antimicrobial resistance to 13 antibiotics was tested using disc diffusion method on Muller Hinton II agar plates. MALDI TOF MS analyses showed lower diversity of bacteria associated to plastic in comparison to sediment and seawater samples. The majority of bacteria from plastic litter belonged to the Vibrio genera with exceptional dominance of V. alginolyticus, a well known opportunistic pathogen in aquaculture that can also cause infections in humans. Conversely, the content of Vibrio spp was generally lower in sediment and water. Antibiotic resistance to ampicillin which is inherent to Vibrio spp, was prevalent among plastic- associated V. alginolyticus, while multiresistant strains occurred occasionally. The results of this study suggest that plastic litter may provide a suitable hotspot surface for the attachment of plastic-specific microbial colonizers. Our findings provide new insights about plastic litter as an important microbial niche in the marine environment.

Izvorni jezik
Engleski

Znanstvena područja
Geologija, Biologija, Interdisciplinarne prirodne znanosti, Veterinarska medicina

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