Pregled bibliografske jedinice broj: 1198334
Deregulated miR-20a/miR-92b circuit underlies changes in circulating γδT cell proportions in psoriasis vulgaris
Deregulated miR-20a/miR-92b circuit underlies changes in circulating γδT cell proportions in psoriasis vulgaris // HLA: Immune Response Genetics Special Issue: Abstracts for the Joint 35th European Immunogenetics and Histocompatibility Conference Amsterdam, the Netherlands, May 17–20, 2022
Amsterdam, Nizozemska, 2022. str. 536-536 doi:10.1111/tan.14606 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 1198334 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Deregulated miR-20a/miR-92b circuit underlies
changes in circulating γδT cell proportions in
psoriasis vulgaris
Autori
Jirouš, Maja ; Štefanić, Mario ; Plužarić, Vera ; Mihalj, Martina ; Grujin, Valentina ; Tolušić Levak, Maja ; Šola, Marija ; Viljetić, Barbara ; Opačak Bernardi, Teuta ; Balogh, Peter ; Tokić, Stana
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
HLA: Immune Response Genetics Special Issue: Abstracts for the Joint 35th European Immunogenetics and Histocompatibility Conference Amsterdam, the Netherlands, May 17–20, 2022
/ - , 2022, 536-536
Skup
36th European Immunogenetics and Histocompatibility Conference
Mjesto i datum
Amsterdam, Nizozemska, 17.05.2022. - 20.05.2022
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
psoriasis, gamma-delta T cells, miR-92b, miR-20a
Sažetak
Psoriasis vulgaris (PV) is a common, autoinflammatory dermatosis driven by T cells. The role played by unconventional, innate-like γδT cell populations in PV has been less well recognised, but the next generation of multi- messenger biology provides an opportunity to break this impasse. To this end, we investigated the composition of flow-sorted γδT cells (CD3ε, panTCRγδ, TCRVδ1/δ2, FACS Canto II/BioRadS3e) in relation to their microRNA expression (hsa-miR- 20a-5p/-29a-3p/-92b-5p, qPCR, reference: hsa-miR- 423-3p, 2-ΔΔCt) by using a deeply phenotyped cohort of 12 affected and 14 control individuals. We also analyzed gene expression in bulk γδT cells (EOMES, RUNX3, TBX21, RORC, CCR6, ZBTB16, SELPLG and IL18R, qPCR, reference: TBP, 2-ΔΔCt) and serum levels of IL-17A/F, IL-18, IL-23, CCL20, and CCL27 (Luminex). Vδ1-δ2- (median 35 % vs. 17 %, P=0.039, Wilcoxon test) and Vδ1-δ2- γδTCRint compartments (40 % vs. 22 %, P=0.02) were expanded in γδT cells from PV patients. The bulk γδT cells in PV were enriched in miR-92b (FC=13.2, P=0.013), but depleted of miR-20a (FC=0.27, P=0.0014). A strong inverse relationship was also noted between miR-20a abundance and the number of Vδ1-δ2- γδTCRint cells in the blood (ρ=-0.61, P=0.0035, Spearman test). ZBTB16, SELPLG and IL18R transcripts followed miR- 20a dynamics (0.44<ρ<0.56, 0.038>P>0.0054), suggesting that cell homing and trafficking may underlie numerical evolution of certain γδT subsets in PV. In contrast, miR-92b lacked association with cell numbers. Age, sex, BMI, the burden of inflammation and CMV status had no major effect on miR-20a/92b expression in bulk γδT cells. In conclusion, we provide an updated constraint on miR-20a/29a/92b expression in purified γδT blood cells from PV donors. A further study into the cellular landscape of miRNA expression is warranted.
Izvorni jezik
Engleski
Znanstvena područja
Biologija, Interdisciplinarne prirodne znanosti
POVEZANOST RADA
Projekti:
HRZZ-UIP-2019-04-3494 - NGS analiza transkriptoma MAIT i yd T limfocita: fenotip, funkcija i raznolikost TCR klonova u pozadini razvoja vulgarne psorijaze (NGSmyPHENOTIP) (Tokić, Stana, HRZZ - HRZZ UIP-2019-04) ( CroRIS)
MZO Ustanova-Medicinski fakultet, Osijek-VIF2018-MEFOS-08 - Uloga microRNA transkriptoma MAIT i γδ T limfocita u razvoju i progresiji vulgarne psorijaze (Tokić, Stana, MZO Ustanova - VIF2018-MEFOS) ( CroRIS)
Ustanove:
Klinički bolnički centar Osijek,
Medicinski fakultet, Osijek
Profili:
Maja Jirouš
(autor)
Marija Šola
(autor)
Maja Tolušić Levak
(autor)
Stana Tokić
(autor)
Teuta Opačak-Bernardi
(autor)
Martina Mihalj
(autor)
Mario Štefanić
(autor)
Barbara Viljetić
(autor)