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Pregled bibliografske jedinice broj: 1198334

Deregulated miR-20a/miR-92b circuit underlies changes in circulating γδT cell proportions in psoriasis vulgaris


Jirouš, Maja; Štefanić, Mario; Plužarić, Vera; Mihalj, Martina; Grujin, Valentina; Tolušić Levak, Maja; Šola, Marija; Viljetić, Barbara; Opačak Bernardi, Teuta; Balogh, Peter; Tokić, Stana
Deregulated miR-20a/miR-92b circuit underlies changes in circulating γδT cell proportions in psoriasis vulgaris // HLA: Immune Response Genetics Special Issue: Abstracts for the Joint 35th European Immunogenetics and Histocompatibility Conference Amsterdam, the Netherlands, May 17–20, 2022
Amsterdam, Nizozemska, 2022. str. 536-536 doi:10.1111/tan.14606 (poster, međunarodna recenzija, sažetak, znanstveni)


CROSBI ID: 1198334 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Deregulated miR-20a/miR-92b circuit underlies changes in circulating γδT cell proportions in psoriasis vulgaris

Autori
Jirouš, Maja ; Štefanić, Mario ; Plužarić, Vera ; Mihalj, Martina ; Grujin, Valentina ; Tolušić Levak, Maja ; Šola, Marija ; Viljetić, Barbara ; Opačak Bernardi, Teuta ; Balogh, Peter ; Tokić, Stana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
HLA: Immune Response Genetics Special Issue: Abstracts for the Joint 35th European Immunogenetics and Histocompatibility Conference Amsterdam, the Netherlands, May 17–20, 2022 / - , 2022, 536-536

Skup
36th European Immunogenetics and Histocompatibility Conference

Mjesto i datum
Amsterdam, Nizozemska, 17.05.2022. - 20.05.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
psoriasis, gamma-delta T cells, miR-92b, miR-20a

Sažetak
Psoriasis vulgaris (PV) is a common, autoinflammatory dermatosis driven by T cells. The role played by unconventional, innate-like γδT cell populations in PV has been less well recognised, but the next generation of multi- messenger biology provides an opportunity to break this impasse. To this end, we investigated the composition of flow-sorted γδT cells (CD3ε, panTCRγδ, TCRVδ1/δ2, FACS Canto II/BioRadS3e) in relation to their microRNA expression (hsa-miR- 20a-5p/-29a-3p/-92b-5p, qPCR, reference: hsa-miR- 423-3p, 2-ΔΔCt) by using a deeply phenotyped cohort of 12 affected and 14 control individuals. We also analyzed gene expression in bulk γδT cells (EOMES, RUNX3, TBX21, RORC, CCR6, ZBTB16, SELPLG and IL18R, qPCR, reference: TBP, 2-ΔΔCt) and serum levels of IL-17A/F, IL-18, IL-23, CCL20, and CCL27 (Luminex). Vδ1-δ2- (median 35 % vs. 17 %, P=0.039, Wilcoxon test) and Vδ1-δ2- γδTCRint compartments (40 % vs. 22 %, P=0.02) were expanded in γδT cells from PV patients. The bulk γδT cells in PV were enriched in miR-92b (FC=13.2, P=0.013), but depleted of miR-20a (FC=0.27, P=0.0014). A strong inverse relationship was also noted between miR-20a abundance and the number of Vδ1-δ2- γδTCRint cells in the blood (ρ=-0.61, P=0.0035, Spearman test). ZBTB16, SELPLG and IL18R transcripts followed miR- 20a dynamics (0.44<ρ<0.56, 0.038>P>0.0054), suggesting that cell homing and trafficking may underlie numerical evolution of certain γδT subsets in PV. In contrast, miR-92b lacked association with cell numbers. Age, sex, BMI, the burden of inflammation and CMV status had no major effect on miR-20a/92b expression in bulk γδT cells. In conclusion, we provide an updated constraint on miR-20a/29a/92b expression in purified γδT blood cells from PV donors. A further study into the cellular landscape of miRNA expression is warranted.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Interdisciplinarne prirodne znanosti



POVEZANOST RADA


Projekti:
HRZZ-UIP-2019-04-3494 - NGS analiza transkriptoma MAIT i yd T limfocita: fenotip, funkcija i raznolikost TCR klonova u pozadini razvoja vulgarne psorijaze (NGSmyPHENOTIP) (Tokić, Stana, HRZZ - HRZZ UIP-2019-04) ( CroRIS)
MZO Ustanova-Medicinski fakultet, Osijek-VIF2018-MEFOS-08 - Uloga microRNA transkriptoma MAIT i γδ T limfocita u razvoju i progresiji vulgarne psorijaze (Tokić, Stana, MZO Ustanova - VIF2018-MEFOS) ( CroRIS)

Ustanove:
Klinički bolnički centar Osijek,
Medicinski fakultet, Osijek

Poveznice na cjeloviti tekst rada:

doi onlinelibrary.wiley.com

Citiraj ovu publikaciju:

Jirouš, Maja; Štefanić, Mario; Plužarić, Vera; Mihalj, Martina; Grujin, Valentina; Tolušić Levak, Maja; Šola, Marija; Viljetić, Barbara; Opačak Bernardi, Teuta; Balogh, Peter; Tokić, Stana
Deregulated miR-20a/miR-92b circuit underlies changes in circulating γδT cell proportions in psoriasis vulgaris // HLA: Immune Response Genetics Special Issue: Abstracts for the Joint 35th European Immunogenetics and Histocompatibility Conference Amsterdam, the Netherlands, May 17–20, 2022
Amsterdam, Nizozemska, 2022. str. 536-536 doi:10.1111/tan.14606 (poster, međunarodna recenzija, sažetak, znanstveni)
Jirouš, M., Štefanić, M., Plužarić, V., Mihalj, M., Grujin, V., Tolušić Levak, M., Šola, M., Viljetić, B., Opačak Bernardi, T., Balogh, P. & Tokić, S. (2022) Deregulated miR-20a/miR-92b circuit underlies changes in circulating γδT cell proportions in psoriasis vulgaris. U: HLA: Immune Response Genetics Special Issue: Abstracts for the Joint 35th European Immunogenetics and Histocompatibility Conference Amsterdam, the Netherlands, May 17–20, 2022 doi:10.1111/tan.14606.
@article{article, author = {Jirou\v{s}, Maja and \v{S}tefani\'{c}, Mario and Plu\v{z}ari\'{c}, Vera and Mihalj, Martina and Grujin, Valentina and Tolu\v{s}i\'{c} Levak, Maja and \v{S}ola, Marija and Viljeti\'{c}, Barbara and Opa\v{c}ak Bernardi, Teuta and Balogh, Peter and Toki\'{c}, Stana}, year = {2022}, pages = {536-536}, DOI = {10.1111/tan.14606}, keywords = {psoriasis, gamma-delta T cells, miR-92b, miR-20a}, doi = {10.1111/tan.14606}, title = {Deregulated miR-20a/miR-92b circuit underlies changes in circulating γδT cell proportions in psoriasis vulgaris}, keyword = {psoriasis, gamma-delta T cells, miR-92b, miR-20a}, publisherplace = {Amsterdam, Nizozemska} }
@article{article, author = {Jirou\v{s}, Maja and \v{S}tefani\'{c}, Mario and Plu\v{z}ari\'{c}, Vera and Mihalj, Martina and Grujin, Valentina and Tolu\v{s}i\'{c} Levak, Maja and \v{S}ola, Marija and Viljeti\'{c}, Barbara and Opa\v{c}ak Bernardi, Teuta and Balogh, Peter and Toki\'{c}, Stana}, year = {2022}, pages = {536-536}, DOI = {10.1111/tan.14606}, keywords = {psoriasis, gamma-delta T cells, miR-92b, miR-20a}, doi = {10.1111/tan.14606}, title = {Deregulated miR-20a/miR-92b circuit underlies changes in circulating γδT cell proportions in psoriasis vulgaris}, keyword = {psoriasis, gamma-delta T cells, miR-92b, miR-20a}, publisherplace = {Amsterdam, Nizozemska} }

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