Pregled bibliografske jedinice broj: 1197943
Long-read sequencing reveals the evolutionary drivers of intra-host diversity across natural RNA mycovirus infections
Long-read sequencing reveals the evolutionary drivers of intra-host diversity across natural RNA mycovirus infections // Virus evolution, 7 (2021), 1-14 doi:10.1093/ve/veab101 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 1197943 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Long-read sequencing reveals the evolutionary drivers of intra-host diversity across natural RNA mycovirus infections
Autori
Leigh, Deborah M. ; Peranić, Karla ; Prospero, Simone ; Cornejo, Carolina ; Ćurković-Perica, Mirna ; Kupper, Quirin ; Nuskern, Lucija ; Rigling, Daniel ; Ježić, Marin
Izvornik
Virus evolution (2057-1577) 7
(2021);
1-14
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
RNA virus, virus evolution, natural infection, PacBio HiFi, intra-host diversity, epistasis, chestnut blight
Sažetak
Intra-host dynamics are a core component of virus evolution but most intra-host data come from a narrow range of hosts or experimental infections. Gaining broader information on the intra-host diversity and dynamics of naturally occurring virus infections is essential to our understanding of evolution across the virosphere. Here we used PacBio long-read HiFi sequencing to characterize the intra-host populations of natural infections of the RNA mycovirus Cryphonectria hypovirus 1 (CHV1). CHV1 is a biocontrol agent for the chestnut blight fungus (Cryphonectria parasitica), which co-invaded Europe alongside the fungus. We characterized the mutational and haplotypic intra-host virus diversity of thirty-eight natural CHV1 infections spread across four locations in Croatia and Switzerland. Intra-host CHV1 diversity values were shaped by purifying selection and accumulation of mutations over time as well as epistatic interactions within the host genome at defense loci. Geographical landscape features impacted CHV1 inter-host relationships through restricting dispersal and causing founder effects. Interestingly, a small number of intra-host viral haplotypes showed high sequence similarity across large geographical distances unlikely to be linked by dispersal.
Izvorni jezik
Engleski
Znanstvena područja
Biologija
POVEZANOST RADA
Projekti:
HRZZ-IZHRZ0_180651 - Dynamics of virus infection in mycovirus-mediated biological control of a fungal pathogen (DynaMyco) (Ježić, Marin, HRZZ - Croatian-Swiss Research Programme 2017 - 2023) ( CroRIS)
Ustanove:
Prirodoslovno-matematički fakultet, Zagreb
Profili:
Mirna Ćurković Perica
(autor)
Marin Ježić
(autor)
Karla Peranić
(autor)
Lucija Nuskern Karaica
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus