Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 1197705

Next-generation sequencing and comparative genomics of 'Candidatus Phytoplasma solani' strains: towards understanding of versatile pathogens


Šeruga Musić, Martina; Drčelić, Marina; Pei, Shen-Chian; Kuo, Chih-Horng
Next-generation sequencing and comparative genomics of 'Candidatus Phytoplasma solani' strains: towards understanding of versatile pathogens // Book of Abstracts: "7th Croatian Congress of Microbiology with International Participation" / Sviličić Petrić, Ines ; Leboš Pavunc, Andreja ; Šantić, Marina ; Kifer, Domagoj (ur.).
Lahti: Hrvatsko mikrobiološko društvo, 2022. str. 48-48 (predavanje, međunarodna recenzija, sažetak, znanstveni)


CROSBI ID: 1197705 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Next-generation sequencing and comparative genomics of 'Candidatus Phytoplasma solani' strains: towards understanding of versatile pathogens

Autori
Šeruga Musić, Martina ; Drčelić, Marina ; Pei, Shen-Chian ; Kuo, Chih-Horng

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts: "7th Croatian Congress of Microbiology with International Participation" / Sviličić Petrić, Ines ; Leboš Pavunc, Andreja ; Šantić, Marina ; Kifer, Domagoj - Lahti : Hrvatsko mikrobiološko društvo, 2022, 48-48

ISBN
978-953-7778-18-7

Skup
7th Croatian Congress of Microbiology

Mjesto i datum
Sveti Martin na Muri, Hrvatska, 24.05.2022. - 27.05.2022

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
bois noir ; effectors ; genom ; phytoplasma ; stolbur

Sažetak
Endocellular wall-less bacteria known as phytoplasmas (genus ‘Candidatus Phytoplasma’) encompass a diverse group of pathogens affecting numerous plant species and causing significant damage in agriculture worldwide. While economic importance is increasing, studies related to their pathogenicity mechanisms are still hindered due to the inability of cultivation in vitro. Nevertheless, the rise of metagenomic era delivered new tools in finding potential virulence factors and effectors enabling phytoplasmas successful manipulation of their plant and insect hosts. ‘Ca. P. solani’ is a cosmopolitan pathogen with a broad host range transmitted by various polyphagous insect-vectors, and one of the common phytoplasmas found in Croatia. In our previous work, we sequenced a genome of ‘Ca. P. solani’ SA-1 strain. The aim of this study was to obtain more genomic data by sequencing two ‘Ca. P. solani’ strains originating from different plant hosts in order to get insight into the pathogenic potential and the repertoire of putative effectors of this versatile phytoplasma species. Whole plant samples of periwinkle infected with two different ‘Ca. P. solani’ strains (ST19 and STOL) maintained by grafting, were used for generation of libraries sequenced on Illumina MiSeq and MinION nanopore sequencing platforms. Raw reads were mapped to the reference genome and reads originating from periwinkle were filtered out. Moreover, de novo assembly was performed by using Unicycler v0.4.9b followed by annotation of genome drafts and prediction of putative effectors. Whole genome alignment and comparative analyses were also performed. Two draft genomes of 17 (ST19) and 22 (STOL) contigs were generated. Total size of drafts obtained were 647, 316 bp (ST19) and 668, 749 bp (STOL) with longest contigs of 140, 634 bp and 158, 078 bp, respectively. Whole genome alignment with reference SA-1 genome revealed synteny and conserved gene order. However, the abundant presence of repetitive sequences and potential mobile units disabled completion of the assembly. Prediction and search for putative effectors showed the presence of homologues of SAP11 and SAP54 effectors as well as some putative effectors that were specific for each of the strains. Nonetheless, sequencing and comparative genome analyses of ‘Ca. P. solani’ genomes confirmed their highly repetitive nature and the presence of diverse putative effectors as a mechanism for successful adaptation and pathogenicity of this versatile pathogen.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekti:
HRZZ-IP-2019-04-2469 - Strategije patogenosti fitoplazmi: efektori, faktori virulencije i pokretni genetički elementi (PathoPhyto) (Šeruga Musić, Martina, HRZZ - 2019-04) ( CroRIS)

Ustanove:
Prirodoslovno-matematički fakultet, Zagreb


Citiraj ovu publikaciju:

Šeruga Musić, Martina; Drčelić, Marina; Pei, Shen-Chian; Kuo, Chih-Horng
Next-generation sequencing and comparative genomics of 'Candidatus Phytoplasma solani' strains: towards understanding of versatile pathogens // Book of Abstracts: "7th Croatian Congress of Microbiology with International Participation" / Sviličić Petrić, Ines ; Leboš Pavunc, Andreja ; Šantić, Marina ; Kifer, Domagoj (ur.).
Lahti: Hrvatsko mikrobiološko društvo, 2022. str. 48-48 (predavanje, međunarodna recenzija, sažetak, znanstveni)
Šeruga Musić, M., Drčelić, M., Pei, S. & Kuo, C. (2022) Next-generation sequencing and comparative genomics of 'Candidatus Phytoplasma solani' strains: towards understanding of versatile pathogens. U: Sviličić Petrić, I., Leboš Pavunc, A., Šantić, M. & Kifer, D. (ur.)Book of Abstracts: "7th Croatian Congress of Microbiology with International Participation".
@article{article, author = {\v{S}eruga Musi\'{c}, Martina and Dr\v{c}eli\'{c}, Marina and Pei, Shen-Chian and Kuo, Chih-Horng}, year = {2022}, pages = {48-48}, keywords = {bois noir, effectors, genom, phytoplasma, stolbur}, isbn = {978-953-7778-18-7}, title = {Next-generation sequencing and comparative genomics of 'Candidatus Phytoplasma solani' strains: towards understanding of versatile pathogens}, keyword = {bois noir, effectors, genom, phytoplasma, stolbur}, publisher = {Hrvatsko mikrobiolo\v{s}ko dru\v{s}tvo}, publisherplace = {Sveti Martin na Muri, Hrvatska} }
@article{article, author = {\v{S}eruga Musi\'{c}, Martina and Dr\v{c}eli\'{c}, Marina and Pei, Shen-Chian and Kuo, Chih-Horng}, year = {2022}, pages = {48-48}, keywords = {bois noir, effectors, genom, phytoplasma, stolbur}, isbn = {978-953-7778-18-7}, title = {Next-generation sequencing and comparative genomics of 'Candidatus Phytoplasma solani' strains: towards understanding of versatile pathogens}, keyword = {bois noir, effectors, genom, phytoplasma, stolbur}, publisher = {Hrvatsko mikrobiolo\v{s}ko dru\v{s}tvo}, publisherplace = {Sveti Martin na Muri, Hrvatska} }




Contrast
Increase Font
Decrease Font
Dyslexic Font