Naslov
Comprehensive degradation study of lipoglycodepsipeptide antibiotic ramoplanin by liquid chromatography and mass spectrometry

Autori
Stražić Novaković, Dubravka ; Galić, Nives

Izvornik
Talanta open (2666-8319) 5 (2022); 100118-100125

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
liquid chromatography, stability indicating method, polypeptide antibiotic, mass spectrometry, forced degradation

Sažetak
Development of new antibiotics is of the utmost importance in the battle against antimicrobial resistance. One of the promising candidates in the fight against resistant bacteria is ramoplanin, a cyclic lipoglycodepsipeptide derived from a fermentation broth. In order to obtain effective and safe pharmaceutical product on the market, development of reliable analytical method for the evaluation of ramoplanin’s purity is vital. In the present work, an LC method was developed, validated and used during stability testing of ramoplanin, in solid state and in solution. Forced degradation study was performed by subjecting the sample to different stress conditions (acid and base hydrolysis, oxidation, humidity, heat and light) and the observed degradation products were elucidated using mass spectrometry. The study revealed significant degradation of ramoplanin under alkaline conditions, leading to formation of lacton hydrolysis product. The results of the study clearly demonstrate that the hydrolysis of the lacton ring is predominant path of ramoplanin’s degradation. However, other significant degradation routs were also observed. Based on the MS/MS results, degradation pathways of ramoplanin are proposed.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA