Naslov
Histomorphometric evaluation of toxic effects of Na-PCP on the gill of the freshwater snail Holandriana holandrii

Autori
Gračan, Romana ; Lajtner, Jasna ; Špoljar, Maria ; Tkalčec, Ines ; Kovačević, Goran ; Banjad Ostojić, Blaženka ; Ivšić, Martina ; Miletić, Ana ; Erben, Radovan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
4th CROATIAN MICROSCOPY CONGRESS with international participation: Book of Abstracts / Macan, Jelena ; Kovačević, Goran - Zagreb : Hrvatsko mikroskopijsko društvo ; Institut Ruđer Bošković, 2022, 71-72

ISBN
978-953-7941-41-3

Skup
4th Croatian Microscopy Congress (CMC 2022)

Mjesto i datum
Poreč, Hrvatska, 18.05.2022. - 20.05.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
gill ; tissue and cell morphometry ; vacuolisation ; necrosis ; image analysis

Sažetak
Compounds of pentachlorophenol and particularly the sodium salt (Na-PCP), have been widely used for a variety of agricultural and industrial purposes. The pollution of aquatic ecosystems by Na-PCP has assumed serious proportions due to its toxicity and accumulative effect in organisms and environment. The objective of this laboratory study was to examine and quantify the histopathological effects of Na-PCP on the structure of the gill, as one of the most important organs targeted by phenol entries, in the freshwater snail, Holandriana holandrii. Based on the results of a preliminary mortality test, three experimental concentrations were chosen: 0.56, 1.00 and 5.6 mg L-1 Na-PCP for the semi-static test. Histological sections, stained with hematoxylin and eosin, were observed under a light microscope equipped with digital camera and analysed with image analysis software to quantify histopathological lesions. Results indicated serious structural changes in epithelial cells and underlaying connective tissue of gills, which were accelerated with an increase of Na-PCP concentrations and more pronounced in relation to the exposed period. Significant gill structural changes appeared as a progressive necrosis at epithelial cells and the epithelial lining was interrupted, while connective tissue exhibited vacuolization, formation of large irregular spaces and damaged chitinous rods. Epithelial height measured showed significant difference among experimental groups exposed to different concentrations of Na-PCP and control. Further on, measured connective tissue width also showed a statistically significant difference. We can conclude that the histomorphometric approach in quantifying of toxicant effects on animal tissue could be applied in legislative prevention of adverse effects in freshwater ecosystems, and H. holandrii can serve as a good indicator of freshwater deterioration.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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