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Pregled bibliografske jedinice broj: 1194864

Characterization of novel imidazolium oximes as selective reactivators of nerve agent-inhibited butyrylcholinesterase


Kolić, Dora; Lulić, Ana-Marija; Spahić, Zlatan; Primožič, Ines; Katalinić, Maja; Kovarik, Zrinka
Characterization of novel imidazolium oximes as selective reactivators of nerve agent-inhibited butyrylcholinesterase // 17th International Symposium on Cholinergic Mechanisms (ISCM2022) - Programme and Abstracts / Kovarik, Zrinka ; Primožič, Ines (ur.).
Zagreb: Institut za medicinska istraživanja i medicinu rada, 2022. str. 62-62 (poster, međunarodna recenzija, sažetak, znanstveni)


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Naslov
Characterization of novel imidazolium oximes as selective reactivators of nerve agent-inhibited butyrylcholinesterase

Autori
Kolić, Dora ; Lulić, Ana-Marija ; Spahić, Zlatan ; Primožič, Ines ; Katalinić, Maja ; Kovarik, Zrinka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
17th International Symposium on Cholinergic Mechanisms (ISCM2022) - Programme and Abstracts / Kovarik, Zrinka ; Primožič, Ines - Zagreb : Institut za medicinska istraživanja i medicinu rada, 2022, 62-62

ISBN
978-953-96817-8-2

Skup
17th International Symposium on Cholinergic Mechanisms (ISCM2022)

Mjesto i datum
Dubrovnik, Hrvatska, 08.05.2022. - 12.05.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
acetylcholinesterase, butyrylcholinesterase, bioscavenging, nerve agents, imidazole oximes

Sažetak
Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) are enzymes crucial for neurotransmitter acetylcholine metabolism in both central and peripheral nervous system. The progressive inhibition of these enzymes by nerve agents (NA) during poisoning disrupts acetylcholine breakdown, leading to excessive impulse transmission and cholinergic crisis. Since BChE circulates freely in the bloodstream, it has been considered as a possible exogenous bioscavenging enzyme which would act as an oxime- assisted catalytic scavenger of NAs, neutralising them before they reach target tissues. Oximes are used in standard clinical therapy to recover activity of inhibited cholinesterases, but reactivating BChE with standard pyridinium oximes proved to be ineffective as they are mainly AChE reactivators. To approach this issue, based on previous research we synthesized eight novel N- benzyl supstituted imidazolium oximes to be considered as reactivators of nerve agent sarin-, cyclosarin- and tabun-inhibited human AChE and BChE. Two oximes emerged as effective reactivators of all three NA-BChE conjugates, possessing superior overall reactivation rates (kr) than standard oxime HI-6 which was up to 3000-times higher for cyclosarin conjugate and 210-times for sarin conjugate, or 100-times higher than oxime TMB-4 in case of tabun conjugate. Binding affinities of phosphylated BChE for novel oximes in terms of 1/KOX increased up to 1900-fold compared to standard oximes and they achieved their reactivation maximum in a shorter time frame. However, standard pyridinium oximes remain better reactivators of phosphylated AChE which indicates that imidazolium oximes with 1, 3- aromatic substituents do not position optimally into the AChE active site gorge. Novel oximes' low toxicity on hepatic (HepG2) and neuronal (SH-SY5Y) cell lines and high capacity for reactivation of BChE in the studied concentration range establishes them as promising candidates for future treatment development.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Temeljne medicinske znanosti, Farmacija



POVEZANOST RADA


Projekti:
IP-2018-01-7683 - Analiza interakcija butirilkolinesteraze s novim inhibitorima i reaktivatorima (AnalyseBChE) (Kovarik, Zrinka, HRZZ - 2018-01) ( CroRIS)
HRZZ-IP-2016-06-3775 - Aktivnošću i in silico usmjeren dizajn malih bioaktivnih molekula (ADESIRE) (Hrenar, Tomica, HRZZ - 2016-06) ( CroRIS)
UIP-2017-05-7260 - MOLEKULARNI MEHANIZMI TOKSIČNOSTI PROTUOTROVA I POTENCIJALNIH LIJEKOVA (CellToxTargets) (Katalinić, Maja, HRZZ - 2017-05) ( CroRIS)

Ustanove:
Institut za medicinska istraživanja i medicinu rada, Zagreb,
Prirodoslovno-matematički fakultet, Zagreb


Citiraj ovu publikaciju:

Kolić, Dora; Lulić, Ana-Marija; Spahić, Zlatan; Primožič, Ines; Katalinić, Maja; Kovarik, Zrinka
Characterization of novel imidazolium oximes as selective reactivators of nerve agent-inhibited butyrylcholinesterase // 17th International Symposium on Cholinergic Mechanisms (ISCM2022) - Programme and Abstracts / Kovarik, Zrinka ; Primožič, Ines (ur.).
Zagreb: Institut za medicinska istraživanja i medicinu rada, 2022. str. 62-62 (poster, međunarodna recenzija, sažetak, znanstveni)
Kolić, D., Lulić, A., Spahić, Z., Primožič, I., Katalinić, M. & Kovarik, Z. (2022) Characterization of novel imidazolium oximes as selective reactivators of nerve agent-inhibited butyrylcholinesterase. U: Kovarik, Z. & Primožič, I. (ur.)17th International Symposium on Cholinergic Mechanisms (ISCM2022) - Programme and Abstracts.
@article{article, author = {Koli\'{c}, Dora and Luli\'{c}, Ana-Marija and Spahi\'{c}, Zlatan and Primo\v{z}i\v{c}, Ines and Katalini\'{c}, Maja and Kovarik, Zrinka}, year = {2022}, pages = {62-62}, keywords = {acetylcholinesterase, butyrylcholinesterase, bioscavenging, nerve agents, imidazole oximes}, isbn = {978-953-96817-8-2}, title = {Characterization of novel imidazolium oximes as selective reactivators of nerve agent-inhibited butyrylcholinesterase}, keyword = {acetylcholinesterase, butyrylcholinesterase, bioscavenging, nerve agents, imidazole oximes}, publisher = {Institut za medicinska istra\v{z}ivanja i medicinu rada}, publisherplace = {Dubrovnik, Hrvatska} }
@article{article, author = {Koli\'{c}, Dora and Luli\'{c}, Ana-Marija and Spahi\'{c}, Zlatan and Primo\v{z}i\v{c}, Ines and Katalini\'{c}, Maja and Kovarik, Zrinka}, year = {2022}, pages = {62-62}, keywords = {acetylcholinesterase, butyrylcholinesterase, bioscavenging, nerve agents, imidazole oximes}, isbn = {978-953-96817-8-2}, title = {Characterization of novel imidazolium oximes as selective reactivators of nerve agent-inhibited butyrylcholinesterase}, keyword = {acetylcholinesterase, butyrylcholinesterase, bioscavenging, nerve agents, imidazole oximes}, publisher = {Institut za medicinska istra\v{z}ivanja i medicinu rada}, publisherplace = {Dubrovnik, Hrvatska} }




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