Naslov
Antimicrobial susceptibility, phylogenetic and proteomic characterization of Enterococcus cecorum

Autori
Lozica, Liča ; Budicin, Emanuel ; Sertić, Sunčica ; Kazazić, Snježana ; Lukač, Maja ; Horvatek Tomić, Danijela ; Gottstein, Željko

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
Peradarski dani 2022 : zbornik = Poultry Days 2022 : proceedings / Balenović, Mirta - Zagreb : Hrvatski veterinarski institut ; Centar za peradarstvo, 2022, 102-108

Skup
XIV. znanstveno-stručni simpozij „PERADARSKI DANI 2019.“ s međunarodnim sudjelovanjem

Mjesto i datum
Poreč, Hrvatska, 11.05.2022. - 14.05.2022

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Enterococcus cecorum ; perad ; disk difuzijska metoda ; AMR ; MALDI-TOF ; 16S
(Enterococcus cecorum ; poultry ; disk diffusion ; AMR ; MALDI-TOF ; 16S)

Sažetak
Pathogenic strains of Enterococcus cecorum are often isolated in broilers and broiler breeders diagnosed with vertebral osteomyelitis, spondylitis or arthritis. The infection is usually characterised by paralysis caused by spinal cord compression of the inflamed sixth thoracic vertebrae. In this study, 16 E. cecorum strains from two poultry farms were analysed. The aim was to determine the phylogenetic and proteomic heterogeneity of the strains and their susceptibility to antimicrobials that can be used in poultry production. Identification of the bacteria was confirmed using Bruker Microflex LT MALDI-TOF Mass Spectrometry, after which the proteomic phenotypes of the investigated strains were compared using the principal component analysis generated with MALDI Biotyper. Antimicrobial susceptibility to eight antimicrobials was determined using disk diffusion assay on blood agar in microaerophilic conditions at 37 °C. The isolates were additionally phylogenetically analysed based on the 16S rRNA sequences, and the phylogenetic tree was generated by Maximum Likelihood method and Kimura 2- parameter model using MEGA X. All strains were resistant to trimethoprim/sulfamethoxazole, while 85.71% and 42.86% of the strains from Farm B were also resistant to norfloxacin and enrofloxacin, respectively. Phylogenetic analysis showed 100% identity match between 75% of the analysed strains, confirming the previous reports on high homogeneity of the pathogenic E. cecorum strains. Proteomic analysis showed more discriminatory results, but additionally confirmed high similarity of the strains regardless of the farm of origin. As both farms supply the birds from the same hatchery, the results of the study could indicate probable infection of the birds shortly after hatching. High resistance to tested antimicrobials also indicates possible early transmission of the strains, as studied farms rarely use antimicrobials. Results of the research confirm presence of the highly virulent strains, their resistance to antimicrobials and the need for continuous monitoring of E. cecorum on broiler farms to reduce its impact on broiler performance and quality.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA