Naslov
PCL/Si-doped multi-phase calcium phosphate scaffolds derived from cuttlefish bone

Autori
Ressler, Antonia ; Bauer, Leonard ; Prebeg, Teodora ; Ledinski, Maja ; Hussainova, Irina ; Urlić, Inga ; Ivanković, Marica ; Ivanković, Hrvoje

Izvornik
Materials (1996-1944) 15 (2022), 9; 3348, 16

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
biogenic source ; biomimetic ; bone scaffold ; calcium phosphate ; calcium silicate ; silicon

Sažetak
Increasing attention is focused on developing biomaterials as temporary scaffolds that provide a specific environment and microstructure for bone tissue regeneration. The aim of the present work was to synthesize silicon-doped biomimetic multi-phase composite scaffolds based on bioactive inorganic phases and biocompatible polymers (poly(ε-caprolactone), PCL) using simple and inexpensive methods. Porous multi-phase composite scaffolds from cuttlefish bone were synthesized using a hydrothermal method and were further impregnated with (3-aminopropyl)triethoxysilane 1–4 times, heat-treated (1000 °C) and coated with PCL. The effect of silicon doping and the PCL coating on the microstructure and mechanical and biological properties of the scaffolds has been investigated. Multi-phase scaffolds based on calcium phosphate (hydroxyapatite, α-tricalcium phosphate, β-tricalcium phosphate) and calcium silicate (wollastonite, larnite, dicalcium silicate) phases were obtained. Elemental mapping revealed homogeneously dispersed silicon throughout the scaffolds, whereas silicon doping increased bovine serum albumin protein adsorption. The highly porous structure of cuttlefish bone was preserved with a composite scaffold porosity of ~78%. A compressive strength of ~1.4 MPa makes the obtained composite scaffolds appropriate for non-load-bearing applications. Cytocompatibility assessment by an MTT assay of human mesenchymal stem cells revealed the non-cytotoxicity of the obtained scaffolds.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo, Temeljne tehničke znanosti

POVEZANOST RADA