Naslov
Procjena biomarkera u krvi ptića bijele rode s različito zagađenih područja u Hrvatskoj
(No harm done: biomarker assessment in white stork nestlings' blood from differently polluted areas in Croatia)

Autori
Bjedov, Dora ; Velki, Mirna ; Mikuška, Tibor ; Kovačić, Lucija Sara ; Jurinović, Luka ; Mikuška, Alma

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
6. Simpozij studenata doktorskih studija PMF-a = 6th Faculty of Science PhD student symposium : knjiga sažetaka = book of abstracts / Schneider, Petra - : Prirodoslovno-matematički fakultet Sveučilišta u Zagrebu, 2022, 188-189

ISBN
978-953-6076-93-2

Skup
6. Simpozij studenata doktorskih studija PMF-a = 6th Faculty of Science PhD Student Symposium

Mjesto i datum
Zagreb, Hrvatska, 23.04.2022. - 24.04.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Recenziran

Ključne riječi
bijela roda ; krv ptića ; procjena biomarkera ; Hrvatska
(blood sampling ; white-stork nestlings ; biomarker analysis ; Croatia)

Sažetak
The white stork (Ciconia ciconia) is an abundant breeding species in continental Croatia, a top predator inhabiting mostly agricultural fields, wetlands, and meadows. White stork nestlings are a good bioindicator species providing information regarding the local environment. Due to environmental transformation and intensive agriculture, white storks leave their breeding areas indicating a decline in natural value. During breeding season 2021, as a part of white stork ringing scheme, blood samples (4 mL) were collected from the nestling brachial vein (n = 106) for enzyme analysis, few feathers were plucked for sex determination, and beak length was measured for determining hatching order. Study areas are in the proximity to three major rivers in Croatia: Sava, Drava and Danube, and one sampling area is located in Zagreb, landfill Jakuševec. A set of biochemical biomarkers: acetylcholinesterase (AChE), carboxylesterase (CES), and glutathione reductase (GR), as well as molecular biomarkers: glutathione (GSH) and reactive oxygen species (ROS) were analysed. By analysing a set of specific and non-specific biomarkers in two blood fractions we obtained a more complete overview of the nestlings' physiological condition. Lower AChE activity is recorded in Podunavlje, an area known for high fertiliser use and intensive agriculture, indicating the presence of chemical stressors in the environment. On the other hand, oxidative stress biomarkers show the lack of statistical difference in regard to sampling areas. Due to complex antioxidative responses, other antioxidative mechanisms may be included, thus no conclusion could be made about pollutants from the environment and/or their possible effect on the nestlings.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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