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Pregled bibliografske jedinice broj: 1192594

Mitochondrial ROS Induce Partial Dedifferentiation of Human Mesothelioma via Upregulation of NANOG

Sedlić, Filip; Seiwerth, Fran; Sepac, Ana; Sikiric, Suncana; Cindric, Marina; Milavic, Marija; Batelja Vuletic, Lovorka; Jakopovic, Marko; Seiwerth, Sven
Mitochondrial ROS Induce Partial Dedifferentiation of Human Mesothelioma via Upregulation of NANOG // Antioxidants, 9 (2020), 7; 606, 17 doi:10.3390/antiox9070606 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 1192594 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Mitochondrial ROS Induce Partial Dedifferentiation of Human Mesothelioma via Upregulation of NANOG

Autori
Sedlić, Filip ; Seiwerth, Fran ; Sepac, Ana ; Sikiric, Suncana ; Cindric, Marina ; Milavic, Marija ; Batelja Vuletic, Lovorka ; Jakopovic, Marko ; Seiwerth, Sven

Izvornik
Antioxidants (2076-3921) 9 (2020), 7; 606, 17

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
pluripotency factors ; reprogramming ; mesothelioma ; reactive oxygen species ; mitochondria

Sažetak
The expression of pluripotency factors is a key regulator of tumor differentiation status and cancer stem cells. The purpose of this study was to examine the expression of pluripotency factors and differentiation status of human mesothelioma and the role of mitochondria in their regulation. We tested the expression of OCT4/POU5F1, NANOG, SOX2, PI3K-AKT pathway and BCL2 genes and proteins in 65 samples of human mesothelioma and 19 samples of normal mesothelium. Mitochondrial membrane potential, reactive oxygen species (ROS) generation and expression of pluripotency factors were also tested in human mesothelioma cell line. Human mesothelium and mesothelioma expressed SOX2, NANOG, PI3K and AKT genes and proteins and POU5F1 gene, whereby NANOG, SOX2 and phosphorylated (activated) AKT were upregulated in mesothelioma. NANOG protein expression was elevated in less differentiated samples of human mesothelioma. The expression of genes of PI3K-AKT pathway correlated with pluripotency factor genes. Mesothelioma cells had functional, but depolarized mitochondria with large capacity to generate ROS. Mitochondrial ROS upregulated NANOG and mitoTEMPO abrogated it. In conclusion, human mesothelioma displays enhanced expression of NANOG, SOX2 and phosphorylated AKT proteins, while elevated NANOG expression correlates with poor differentiation of human mesothelioma. Mitochondria of mesothelioma cells have a large capacity to form ROS and thereby upregulate NANOG, leading to dedifferentiation of mesothelioma.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Kliničke medicinske znanosti



POVEZANOST RADA

Projekti:
IP-2019-04-1449 - Mitohondrijske podpopulacije i UPRmt u dijabetičkoj kardiomiopatiji (SweetMitochondria) (Sedlić, Filip, HRZZ - 2019-04) ( CroRIS)
--IP-2014-09-4173 - Reprogramiranje citoprotektivnih puteva u mezoteliomu (ReprogrammingMM) (Seiwerth, Sven) ( CroRIS)

Ustanove:
Medicinski fakultet, Zagreb

Profili:

Avatar Url Sunčana Sikirić (autor)

Avatar Url Sven Seiwerth (autor)

Avatar Url Lovorka Batelja Vuletić (autor)

Avatar Url Marina Cindrić (autor)

Avatar Url Filip Sedlić (autor)

Avatar Url Marija Milavić (autor)

Avatar Url Marko Jakopović (autor)

Avatar Url Ana Šepac (autor)

Poveznice na cjeloviti tekst rada:

Pristup cjelovitom tekstu rada doi www.mdpi.com www.researchgate.net

Citiraj ovu publikaciju:

Sedlić, Filip; Seiwerth, Fran; Sepac, Ana; Sikiric, Suncana; Cindric, Marina; Milavic, Marija; Batelja Vuletic, Lovorka; Jakopovic, Marko; Seiwerth, Sven
Mitochondrial ROS Induce Partial Dedifferentiation of Human Mesothelioma via Upregulation of NANOG // Antioxidants, 9 (2020), 7; 606, 17 doi:10.3390/antiox9070606 (međunarodna recenzija, članak, znanstveni)
Sedlić, F., Seiwerth, F., Sepac, A., Sikiric, S., Cindric, M., Milavic, M., Batelja Vuletic, L., Jakopovic, M. & Seiwerth, S. (2020) Mitochondrial ROS Induce Partial Dedifferentiation of Human Mesothelioma via Upregulation of NANOG. Antioxidants, 9 (7), 606, 17 doi:10.3390/antiox9070606.
@article{article, author = {Sedli\'{c}, Filip and Seiwerth, Fran and Sepac, Ana and Sikiric, Suncana and Cindric, Marina and Milavic, Marija and Batelja Vuletic, Lovorka and Jakopovic, Marko and Seiwerth, Sven}, year = {2020}, pages = {17}, DOI = {10.3390/antiox9070606}, chapter = {606}, keywords = {pluripotency factors, reprogramming, mesothelioma, reactive oxygen species, mitochondria}, journal = {Antioxidants}, doi = {10.3390/antiox9070606}, volume = {9}, number = {7}, issn = {2076-3921}, title = {Mitochondrial ROS Induce Partial Dedifferentiation of Human Mesothelioma via Upregulation of NANOG}, keyword = {pluripotency factors, reprogramming, mesothelioma, reactive oxygen species, mitochondria}, chapternumber = {606} }
@article{article, author = {Sedli\'{c}, Filip and Seiwerth, Fran and Sepac, Ana and Sikiric, Suncana and Cindric, Marina and Milavic, Marija and Batelja Vuletic, Lovorka and Jakopovic, Marko and Seiwerth, Sven}, year = {2020}, pages = {17}, DOI = {10.3390/antiox9070606}, chapter = {606}, keywords = {pluripotency factors, reprogramming, mesothelioma, reactive oxygen species, mitochondria}, journal = {Antioxidants}, doi = {10.3390/antiox9070606}, volume = {9}, number = {7}, issn = {2076-3921}, title = {Mitochondrial ROS Induce Partial Dedifferentiation of Human Mesothelioma via Upregulation of NANOG}, keyword = {pluripotency factors, reprogramming, mesothelioma, reactive oxygen species, mitochondria}, chapternumber = {606} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus

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