Pregled bibliografske jedinice broj: 1192538
D-mannose-Coating of Maghemite Nanoparticles Improved Labeling of Neural Stem Cells and Allowed Their Visualization by ex vivo MRI after Transplantation in the Mouse Brain
D-mannose-Coating of Maghemite Nanoparticles Improved Labeling of Neural Stem Cells and Allowed Their Visualization by ex vivo MRI after Transplantation in the Mouse Brain // Cell Transplantation, 28 (2019), 5; 553-567 doi:10.1177/0963689719834304 (međunarodna recenzija, članak, znanstveni)
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Naslov
D-mannose-Coating of Maghemite Nanoparticles
Improved Labeling of Neural
Stem Cells and Allowed Their Visualization by ex
vivo MRI after
Transplantation in the Mouse Brain
Autori
Pongrac, Igor M. ; Radmilović, Marina Dobrivojević ; Ahmed, Lada Brkić ; Mlinarić, Hrvoje ; Regul, Jan ; Škokić, Siniša ; Babič, Michal ; Horák, Daniel ; Hoehn, Mathias ; Gajović, Srećko
Izvornik
Cell Transplantation (0963-6897) 28
(2019), 5;
553-567
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
brain ; maghemite ; magnetic resonance imaging ; mouse ; nanoparticles ; neural stem cells
Sažetak
Magnetic resonance imaging (MRI) of superparamagnetic iron oxide- labeled cells can be used as a non-invasive technique to track stem cells after transplantation. The aim of this study was to (1) evaluate labeling efficiency of D- mannose-coated maghemite nanoparticles (D- mannose(γ-Fe2O3)) in neural stem cells (NSCs) in comparison to the uncoated nanoparticles, (2) assess nanoparticle utilization as MRI contrast agent to visualize NSCs transplanted into the mouse brain, and (3) test nanoparticle biocompatibility. D- mannose(γ-Fe2O3) labeled the NSCs better than the uncoated nanoparticles. The labeled cells were visualized by ex vivo MRI and their localization subsequently confirmed on histological sections. Although the progenitor properties and differentiation of the NSCs were not affected by labeling, subtle effects on stem cells could be detected depending on dose increase, including changes in cell proliferation, viability, and neurosphere diameter. D-mannose coating of maghemite nanoparticles improved NSC labeling and allowed for NSC tracking by ex vivo MRI in the mouse brain, but further analysis of the eventual side effects might be necessary before translation to the clinic.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
POVEZANOST RADA
Projekti:
EK-FP7-316120 - Combining Stem Cells and Biomaterials for Brain Repair - Unlocking the Potential of the Existing Brain Research through Innovative In Vivo Molecular Imaging (GLOWBRAIN) (EK - FP7-REGPOT-2012-2013-1) ( CroRIS)
Ustanove:
Medicinski fakultet, Zagreb
Profili:
Srećko Gajović (autor)
Lada Brkić (autor)
Igor Pongrac (autor)
Siniša Škokić (autor)
Marina Radmilović (autor)
Hrvoje Mlinarić (autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE