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Pregled bibliografske jedinice broj: 1192532

Human Neural Stem Cell Induced Functional Network Stabilization After Cortical Stroke: A Longitudinal Resting-State fMRI Study in Mice


Minassian, Anuka; Green, Claudia; Diedenhofen, Michael; Vogel, Stefanie; Hess, Simon; Stoeber, Maren; Radmilovic, Marina Dobrivojevic; Wiedermann, Dirk; Kloppenburg, Peter; Hoehn, Mathias
Human Neural Stem Cell Induced Functional Network Stabilization After Cortical Stroke: A Longitudinal Resting-State fMRI Study in Mice // Frontiers in Cellular Neuroscience, 14 (2020), 86, 16 doi:10.3389/fncel.2020.00086 (međunarodna recenzija, članak, znanstveni)


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Naslov
Human Neural Stem Cell Induced Functional Network Stabilization After Cortical Stroke: A Longitudinal Resting-State fMRI Study in Mice
(Human Neural Stem Cell Induced Functional Network Stabilization After Cortical Stroke: A Longitudinal Resting-State fMRI Study in Mice)

Autori
Minassian, Anuka ; Green, Claudia ; Diedenhofen, Michael ; Vogel, Stefanie ; Hess, Simon ; Stoeber, Maren ; Radmilovic, Marina Dobrivojevic ; Wiedermann, Dirk ; Kloppenburg, Peter ; Hoehn, Mathias

Izvornik
Frontiers in Cellular Neuroscience (1662-5102) 14 (2020); 86, 16

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
distal MCA occlusion ; functional connectivity ; human neural stem cells ; moderate severity stroke model ; mouse ; neuronal differentiation ; resting-state fMRI ; stroke-induced hyperconnectivity

Sažetak
Most stroke studies dealing with functional deficits and assessing stem cell therapy produce extensive hemispheric damage and can be seen as a model for severe clinical strokes. However, mild strokes have a better prospect for functional recovery. Recently, anatomic and behavioral changes have been reported for distal occlusion of the middle cerebral artery (MCA), generating a well-circumscribed and small cortical lesion, which can thus be proposed as mild to moderate cortical stroke. Using this cortical stroke model of moderate severity in the nude mouse, we have studied the functional networks with resting-state functional magnetic resonance imaging (fMRI) for 12 weeks following stroke induction. Further, human neural stem cells (hNSCs) were implanted adjacent to the ischemic lesion, and the stable graft vitality was monitored with bioluminescence imaging (BLI). Differentiation of the grafted neural stem cells was analyzed by immunohistochemistry and by patch-clamp electrophysiology. Following stroke induction, we found a pronounced and continuously rising hypersynchronicity of the sensorimotor networks including both hemispheres, in contrast to the severe stroke filament model where profound reduction of the functional connectivity had been reported by us earlier. The vitality of grafted neural stem cells remained stable throughout the whole 12 weeks observation period. In the stem cell treated animals, functional connectivity did not show hypersynchronicity but was globally slightly reduced below baseline at 2 weeks post- stroke, normalizing thereafter completely. Our resting-state fMRI (rsfMRI) studies on cortical stroke reveal for the first time a hypersynchronicity of the functional brain networks. This hypersynchronicity appears as a hallmark of mild cortical strokes, in contrast to severe strokes with striatal involvement where exclusively hyposynchronicity has been reported. The effect of the stem cell graft was an early and persistent normalization of the functional sensorimotor networks across the whole brain. These novel functional results may help interpret future outcome investigations after stroke and demonstrate the highly promising potential of stem cell treatment for functional outcome improvement after stroke.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Ustanove:
Medicinski fakultet, Zagreb

Profili:

Avatar Url Marina Radmilović (autor)

Poveznice na cjeloviti tekst rada:

doi www.frontiersin.org

Citiraj ovu publikaciju:

Minassian, Anuka; Green, Claudia; Diedenhofen, Michael; Vogel, Stefanie; Hess, Simon; Stoeber, Maren; Radmilovic, Marina Dobrivojevic; Wiedermann, Dirk; Kloppenburg, Peter; Hoehn, Mathias
Human Neural Stem Cell Induced Functional Network Stabilization After Cortical Stroke: A Longitudinal Resting-State fMRI Study in Mice // Frontiers in Cellular Neuroscience, 14 (2020), 86, 16 doi:10.3389/fncel.2020.00086 (međunarodna recenzija, članak, znanstveni)
Minassian, A., Green, C., Diedenhofen, M., Vogel, S., Hess, S., Stoeber, M., Radmilovic, M., Wiedermann, D., Kloppenburg, P. & Hoehn, M. (2020) Human Neural Stem Cell Induced Functional Network Stabilization After Cortical Stroke: A Longitudinal Resting-State fMRI Study in Mice. Frontiers in Cellular Neuroscience, 14, 86, 16 doi:10.3389/fncel.2020.00086.
@article{article, author = {Minassian, Anuka and Green, Claudia and Diedenhofen, Michael and Vogel, Stefanie and Hess, Simon and Stoeber, Maren and Radmilovic, Marina Dobrivojevic and Wiedermann, Dirk and Kloppenburg, Peter and Hoehn, Mathias}, year = {2020}, pages = {16}, DOI = {10.3389/fncel.2020.00086}, chapter = {86}, keywords = {distal MCA occlusion, functional connectivity, human neural stem cells, moderate severity stroke model, mouse, neuronal differentiation, resting-state fMRI, stroke-induced hyperconnectivity}, journal = {Frontiers in Cellular Neuroscience}, doi = {10.3389/fncel.2020.00086}, volume = {14}, issn = {1662-5102}, title = {Human Neural Stem Cell Induced Functional Network Stabilization After Cortical Stroke: A Longitudinal Resting-State fMRI Study in Mice}, keyword = {distal MCA occlusion, functional connectivity, human neural stem cells, moderate severity stroke model, mouse, neuronal differentiation, resting-state fMRI, stroke-induced hyperconnectivity}, chapternumber = {86} }
@article{article, author = {Minassian, Anuka and Green, Claudia and Diedenhofen, Michael and Vogel, Stefanie and Hess, Simon and Stoeber, Maren and Radmilovic, Marina Dobrivojevic and Wiedermann, Dirk and Kloppenburg, Peter and Hoehn, Mathias}, year = {2020}, pages = {16}, DOI = {10.3389/fncel.2020.00086}, chapter = {86}, keywords = {distal MCA occlusion, functional connectivity, human neural stem cells, moderate severity stroke model, mouse, neuronal differentiation, resting-state fMRI, stroke-induced hyperconnectivity}, journal = {Frontiers in Cellular Neuroscience}, doi = {10.3389/fncel.2020.00086}, volume = {14}, issn = {1662-5102}, title = {Human Neural Stem Cell Induced Functional Network Stabilization After Cortical Stroke: A Longitudinal Resting-State fMRI Study in Mice}, keyword = {distal MCA occlusion, functional connectivity, human neural stem cells, moderate severity stroke model, mouse, neuronal differentiation, resting-state fMRI, stroke-induced hyperconnectivity}, chapternumber = {86} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


Citati:





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