Naslov
Globularia alypum L. and related species: LC-MS profiles and antidiabetic, antioxidant, anti- inflammatory, antibacterial and anticancer potential

Autori
Friščić, Maja ; Petlevski, Roberta ; Kosalec, Ivan ; Madunić, Josip ; Matulić, Maja ; Bucar, Franz ; Hazler Pilepić, Kroata ; Maleš, Željan

Izvornik
Pharmaceuticals (1424-8247) 15 (2022), 5; 1-34

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Globularia alypum ; metabolite profiling ; hyperglycemia-induced oxidative stress ; antiradical activity ; cyclooxygenase-1 ; well diffusion ; broth microdilution ; MRSA ; MDA-MB-231 ; A1235

Sažetak
Species from the genus Globularia L. have been used as healing agents for various ailments, with utilization of Globularia alypum L. being most frequently reported. The aim of this study was to evaluate the antidiabetic, antioxidant, anti- inflammatory, antibacterial and anticancer potential of G. alypum and three related species, G. punctata Lapeyr., G. cordifolia L. and G. meridionalis (Podp.) O.Schwarz, in relation to their phytochemical compositions. Globularin and verbascoside were identified using LC-PDA-ESI-MSn as the major metabolites of G. alypum with known biological activities. G. alypum demonstrated the greatest α-glucosidase inhibitory activity and DPPH radical scavenging activity (IC50 = 17.25 μg/mL), while its anti- inflammatory activity was not significantly different from those of related species. All investigated species showed considerable antibacterial activity against methicillin-resistant Staphylococcus aureus in the broth microdilution method (MIC = 1.42–3.79 mg/mL). G. punctata also showed antibacterial activities against Escherichia coli (MIC = 1.42 mg/mL), Bacillus subtilis (MIC = 1.89 mg/mL), B. cereus (MIC = 2.84 mg/mL) and Enterococcus faecalis (MBC = 5.68 mg/mL). G. punctata, G. cordifolia and G. meridionalis showed greater anticancer potential than G. alypum. Obtained results indicate investigated Globularia species could serve as sources of diverse bioactive molecules, with G. punctata having the greatest antibacterial potential.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Farmacija

POVEZANOST RADA